Stereocaulon glabrum is a species in the genus Stereocaulon, which is part of the Stereocaulaceae family. This lichen presents distinctive traits and it is a potential source of significant secondary metabolites. Understanding S. glabrum's chemical composition is of importance for investigating its possible applications in disciplines such as pharmaceuticals. Stereocaulon genus is distinguished by its crustose-type primary thallus and fruticose type secondary thallus. This species can be found colonizing numerous substrates, such as rocks, soil, and tree bark, and is distributed across various geographical regions, making it a very good candidate in terms of quantity for future applications. According to earlier research, Stereocaulon genus synthesizes a wide range of secondary metabolites that support the organism's biological functions, chemical interactions with its environment and also has potential in medicinal applications. These metabolites include depsides, depsidones, dibenzofurans, and other specific chemicals. Almost all of them are of phenolic nature displaying a diverse range of chemical structures. The typical secondary metabolites in the Stereocaulon genus are stictic acid, atranorin, lobaric acid, and other phenolic compounds. In order to identify similarities and potential differences in molecular identification, we will compare the compounds that we have identified in S. glabrum to those that are regularly found in other Stereocaulon species. The primary goal of this thesis is to start a comprehensive analysis of the secondary metabolites present in S. glabrum. We will isolate and characterize some of the chemical components present in this lichen by using analytical and spectroscopic methods like chromatography, NMR spectroscopy, and mass spectrometry. We anticipate that the results of this research will advance our knowledge of Stereocaulon genus chemical diversity and pharmacological potential, offering important insights into the identification of new leads for drug develpment, discovery of new natural products, and new physiologically active chemicals in the future.