This thesis shows characterization and identification of microplastic waste found in the Zaglav beach sediment on Vis island. Sampling and separation of samples was performed by the Institute of Oceanography and Fisheries in Split. Characterization of microplastic was performed on 865 specimens from the Zaglav beach on Vis island. Specimens were visually analysed and sorted into categories according to shape defined by DeFishGear protocol: filaments, films, fragments, granules, pellets and foams. Transparency and color of the individual specimens are visually determined and a mass was determined using an analytical balance. Area and maximum dimension were determined using software package Digimizer for image processing. Identification of the type of polymer material was performed by FTIR spectroscoopy and diferential scanning calorimetry, while other smaller specimens were left for the further analysis using more precisely methods. The most common category is fragments, spesimens are mostly white in color and opaque, particles less than 5mm in diameter which sorts them in category of microplastics. The most common type of polymer material is polypropylene, followed by polyethylene high density and polyethylene low density. Results obtained by this research show that most specimens from Zaglav beach originate from improperly discarded disposable packaging.