| Abstract | Ciljevi istraživanja: Kvalitativno određivanje parabena u kozmetičkim proizvodima primjenom GC-MS metode te usporedba dobivenih
rezultata s podatcima deklariranim na proizvodu. Pronaći poveznicu između cijene i prisustva parabena u kozmetičkom proizvodu.
Ustroj istraživanja: eksperimentalna studija
Mjesto istraživanja: Kemijsko-toksikološki laboratorij Kliničkog odjela za sudsku medicinu Zavoda za patologiju, sudsku medicinu i
citologiju, Kliničkog bolničkog centra u Splitu
Materijali i metode: Odabrano je 25 kozmetičkih proizvoda za primjenu u pazužnom području, od toga 15 sprejeva s potisnim plinom, 5
roll-onova i 4 sticka. Od kozmetičkog proizvoda broj 5 dobivena su 2 uzorka: uzorak 5 istovjetnog načina pripreme kao i ostali uzorci te
uzorak 5(2) koji je dodatno maceriran na magnetskoj miješalici. Sprejevi prethodno naneseni na Petrijevu zdjelicu, ostavljeni su na
isparavanje u digestoru te su tako pripremljeni i otpareni otopljeni u kloroformu. Potom je uslijedila filtracija. Naposljetku, pripremljeni su
uzorci prebačeni u staklene tubice za kvalitativnu analizu na GC-MS uređaju. Analiza je izvršena metodom koja omogućava istovremeno
snimanje ukupnog ionskog kromatograma (TIC) u području od 40 – 600 m/z.
Rezultati: Parabeni su detektirani u dvama uzorcima: br. 1 i 4. U uzorku br. 1 pronađeni su: metilparaben, etilparaben, propilparaben i
butilparaben od kojih etilparaben nije deklariran. U uzorku br. 4 pronađen je metilparaben koji je u skladu s navedenom deklaracijom. U
uzorcima br. 1, 9, 17, 22, 24 pronađen je dietilftalat koji nije deklariran ni na jednom od ovih 5 uzoraka. U uzorku br. 5 nisu pronađeni
parabeni (ni ftalati) nakon maceracije. Uvedeni su medijani cijena (Microsoft Excel) cjelokupne skupine proizvoda (14,66 kn), sprejeva
(14,35 kn) i roll-onova (43,98 kn). Uzimajući u obzir zbrojni medijan od 14, 66 kn, 50 % proizvoda pozitivnih na parabene i ftalate nalazi se
iznad, a 50 % ispod medijana. Kod medijana koji vrijedi za roll-onove oba proizvoda (100 %) pripadaju nižem cjenovnom rangu (ispod
medijana). Cijene uzoraka u kojima su identificirani ftalati u iznosu od 60 % pripadaju skupini ispod medijana, odnosno 75 % ne uračunavši
uzorak br. 1 zbog karakterističnog odstupanja cijene roll-ona od dezodoransa u spreju.
Zaključak: Kozmetički proizvodi koji sadrže parabene u većini se slučajeva podudaraju s navedenom deklaracijom dok ftalati u potpunosti
odstupaju. Prema tome, potrebne su strože kontrole utjecaja ambalaže na proizvode niže cjenovne kategorije kod preparata za osobnu njegu.
Kod analize proizvoda u kojima su identificirani parabeni i ftalati, usporedbom s njihovom cijenom, nisu dobiveni nedvojbeni rezultati, stoga
je potrebno provesti detaljnije studije. |
| Abstract (english) | Objectives: Qualitative determination of parabens in cosmetic products using GC-MS method as well as the comparison of the results with
those declared on the products. In addition, another objective was to find a correlation between the price range below the median value and
paraben presence in cosmetic products.
Design: Experimental study
Settings: Laboratory of toxicology, Department of pathology, medicine and cytology, University Hospital of Split
Materials and methods: In this study 25 underarm cosmetics were chosen, more precisely 15 sprays, 5 roll-ons and 4 sticks. The cosmetic
product number 5 was prepared as 2 samples: number 5 was prepared identically to the procedure described, while the 5(2) sample was
additionally stirred. Sprays, previously applied to Petri Dishes, were left to volatilize in the digester. Then, all of the 26 samples were
prepared by dissolving them with chloroform, and followed by filtration. Finally, the samples were transferred to glass cuvettes for the
qualitative analysis using GC-MS method. Analysis was performed by a method that simultaneously records the total ion chromatogram
(TIC) in the area of 40-600 m/z.
Results: Parabens were detected in two samples: number 1 and 4. In sample number 1 methyl paraben, ethyl paraben, propyl paraben and
butyl paraben were identified from which ethyl paraben was not printed on the declaration label. In samples number 1, 9, 17, 22 and 24
diethyl phthalate was determined but was not declared anywhere. Sample number 5 was free from parabens before and after it was stirred.
The 3 price medians were calculated (Microsoft Excel): for the entire personal care products’ group (14.66), only for the sprays (14.35) and
roll-ons (43.98). Taking into consideration the 14.66 median, 50 % of the paraben- and phthalate- positive products are above and 50 %
below the median. Taking just roll-ons into account, both products (100 %) were classified as the lower price range group. 60 % of the
phthalate-positive samples are below price median, or 75 % excluding sample number 1 because of the characteristic price difference
between a deodorant and a roll-on.
Conclusion: Cosmetic products identified with parabens were mostly in accordance with the declaration label. On the other hand, opposite
results were obtained with phthalates. A more reliable control system of packaging influence on the product itself is highly suggested
(especially in the lower price range). The results of the correlation between the prices of the products where parabens and phthalates have
been identified are ambiguous, hence more thorough statistical analysis and studies are required. |
