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master's thesis
Kinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode
diplomski rad
2016. urn:nbn:hr:167:254235
Bitanga, Ana
University of Split
Faculty of Chemistry and Technology

Cite this document

Bitanga, A. (2016). Kinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:254235

Bitanga, Ana. "Kinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:254235

Bitanga, Ana. "Kinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:254235

Bitanga, A. (2016). 'Kinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:254235

Bitanga A. Kinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2016 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:167:254235

A. Bitanga, "Kinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:167:254235

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Metadata
TitleKinetičko spektrofotometrijsko određivanje N-acetil-L-cistein-etil estera: razvoj i vrednovanje metode : diplomski rad
Title (english)Kinetic spectrophotometric determination of N-acetyl-L-cysteine-ethyl ester : method development and validation : master thesis
AuthorAna Bitanga
MentorLea Kukoč Modun (mentor)
Committee memberMila Radan (predsjednik povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
Defense date and country2016-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Cilj istraživanja koje je prikazano u ovom radu je razvoj i vrednovanje kinetičke metode analize N-acetil-L-cistein-etil estera (NACET) uz spektrofotometrijski detektor. Opisana kinetička metoda temelji se na redoks reakciji u kojoj NACET reducira [Cu(BCA)₂]²⁺ kompleks zelene boje u [Cu(BCA)₂]⁺ ljubičasto obojan kompleks čiji se apsorpcijski maksimum bilježi na 562 nm. Optimizirani su parametri kemijske reakcije te je temeljem toga konstruirana krivulja umjeravanja čijom je regresijskom analizom utvrđena jednadžba pravca: y = 5991,8 x + 0,00235. Postignuta je linearnost u području koncentracija od 6,0 x 10⁻⁷ do 1,0 x 10⁻⁴ mol L⁻¹, te su određene granica dokazivanja 1,60 x 10⁻⁷ mol L⁻¹ i granica određivanja 5,45 x 10⁻⁷ mol L⁻¹. Ispitan je i utjecaj pojedinih interferencija na NACET te nije zamijećen nikakav značajan utjecaj na određivanje analita.
Abstract (english)
The main goal of this research is development and validation of kinetic method for the determination of N-Acetylcysteine-ethyl ester (NACET) with spectrophotometric detector. Described kinetic method is based on the redox reaction where NACET reduced [Cu(BCA)₂]²⁺ green complex to the purple [Cu(BCA)₂]⁺ complex with absorption maximum at 562 nm. The parameters of chemical reaction have been optimized and the calibration curve was constructed. Linear dynamic range was from 6,0 x 10⁻⁷ to 1,0 x 10⁻⁴ mol L⁻¹, with equation direction y = 5991,8 x + 0,00235. Limit of detection and limit of quantification were found to be 1,60 x 10⁻⁷ mol L⁻¹ and 5,45 x 10⁻⁷ mol L⁻¹. Impact of certain interferences has been examined and any significant impact on the determination of an analyte has not been noticed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:254235
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Organic Chemistry and Biochemistry
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-09-27 08:37:12