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master's thesis
Kemijski sastav i biološki potencijal motra
diplomski rad
2023. urn:nbn:hr:167:333521
Podrug, Roko
University of Split
Faculty of Chemistry and Technology
Division of Engineering and Chemistry

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Podrug, R. (2023). Kemijski sastav i biološki potencijal motra : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:333521

Podrug, Roko. "Kemijski sastav i biološki potencijal motra : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:333521

Podrug, Roko. "Kemijski sastav i biološki potencijal motra : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:333521

Podrug, R. (2023). 'Kemijski sastav i biološki potencijal motra : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 28 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:333521

Podrug R. Kemijski sastav i biološki potencijal motra : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2023 [cited 2024 December 28] Available at: https://urn.nsk.hr/urn:nbn:hr:167:333521

R. Podrug, "Kemijski sastav i biološki potencijal motra : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:167:333521

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Metadata
TitleKemijski sastav i biološki potencijal motra : diplomski rad
Title (english)CHEMICAL COMPOSITION AND BIOLOGICAL POTENTIAL OF SEA FENNEL : diplom athesis
AuthorRoko Podrug
MentorDanijela Skroza (mentor)
Committee memberIvana Generalić Mekinić (predsjednik povjerenstva)
Committee memberDanijela Skroza (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Engineering and Chemistry)
Split
Defense date and country2023-10-25, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Cilj ovog rada bio je istražiti utjecaj okolišnih faktora na kemijski sastav i biološki potencijal samonikle biljke motar (Crithmum maritimum L.) te mogućnosti njene primjene u prehrambenoj industriji. Analizirani su metanolni ekstrakti lista motra prikupljeni duž Jadranske obale na 7 različitih lokacija. U eksperimentalnom dijelu rada spektrofotometrijski je u uzorcima određen udio ukupnih fenola i flavonoida, te je korištenjem tekućinske kromatografije visoke djelotvornosti (HPLC; engl. High-performance liquid chromatography) identificirana i kvantificirana klorogenska kiselina i njeni derivati. Antioksidacijska aktivnost određena je pomoću 3 različite metode: metodom određivanja snage redukcije željeza (engl. Ferric Reducing Antioxidant Power, FRAP), ispitivanjem kapaciteta apsorpcije kisikovih radikala (engl. Oxygen Radical Absorbance Capacity, ORAC) te metodom redukcije 2,2-difenil-1-pikrilhidrazil radikala (engl. 2,2 diphenyl-1-pycrilhydrazyl, DPPH). Dobiveni rezultati ukazuju na utjecaj mjesta uzorkovanja na njihov kemijski sastav i biološki potencijal. Pjeskovito stanište, koje biljci uskraćuje hranjive tvari i izlaže je stresu, pokazalo se kao potencijalno važni uzročnik bogatog fenolnog sastava i izuzetnog biološkog potencijala uzoraka. Sukladno tome, najviši udio fenolnih komponenti (ukupni fenoli, flavonoidi, klorogenska kiselina) i najbolja redukcijska snaga i antioksidacijski potencijal zabilježen je u uzorku lista motra uzorkovanom na lokaciji Neretva, dok su uzorci prikupljeni na srednjem Jadranu (Lokacije Split i Drašnice) imali najniži udio navedenih spojeva i slabu antioksidacijsku aktivnost.
Abstract (english)
The aim of this work was to investigate the influence of environmental factors on the chemical composition and biological potential of the wild sea fennel (Crithmum maritimum L.) and the possibilities of its application in the food industry. Methanolic extracts of sea fennel leaves collected along the Adriatic coast at 7 different locations were analyzed. In the experimental part of the work, the content of total phenols and flavonoids was determined spectrophotometrically, while the concentration of chlorogenic acid and its derivatives was identified and quantified using high-performance liquid chromatography (HPLC). Antioxidant activity was determined using 3 different methods: the Ferric Reducing Antioxidant Power (FRAP) method, the Oxygen Radical Absorbance Capacity (ORAC) test, and the method of reducing 2,2-diphenyl- 1-picrylhydrazyl radical (DPPH). The obtained results indicate the influence of the sampling site on the chemical composition and biological potential of the samples. The sandy habitat, which deprives the plant of nutrients and exposes it to stress, can potentially be a reason for the rich phenolic composition and exceptional biological potential of the samples. Accordingly, the highest proportion of phenolic components (total phenols, flavonoids, chlorogenic acid) and the best reducing power and antioxidant potential was recorded for the sea fennel leaves sampled at the Neretva location, while the samples collected in the central Adriatic (Split and Drašnice locations) had the lowest content of the mentioned compounds and weak antioxidant activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:333521
Study programmeTitle: Graduate university study of Food technology - graduate - university
Study programme type: university
Study level: graduate
Academic / professional title: magistar/a inženjer/ka prehrambene tehnologije (magistar/a inženjer/ka prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteOvaj rad je u potpunosti financiran sredstvima projekta SEAFENNEL4MED (PRIMA 2021, Section 2) (https://seafennel4med.com/). Dio opreme korištene u ovom radu financiran je iz projekta EU „Funkcionalna integracija Sveučilišta u Splitu, PMF-ST, PFST te KTF-ST kroz razvoj znanstveno-istraživačke infrastrukture u Zgradi tri fakulteta“, KK. 01.1.1.02.0018.
Created on2023-11-10 08:49:54