Glucosinolates are secondary metabolites identified in over 16 different plant families, among which the most important is Brassicaceae family, which includes well known plants of everyday nutrition. Glucosinolates are physiologically inert and chemically stable upon activation of the enzyme myrosinase leads to the degradation of glucosinolates and formation of various compounds. They are considered as phytochemicals which have potential to protect the human body form various diseases. In this diploma thesis, glucosinolates from selected parts of Nasturtium officinale and Iberis umbellata plants were isolated and analysed. Glucosinolates were identified by UHPLC-DAD-MS/MS analyzing desulfated glucosinolates. The same were quantified in different plant parts according to the official ISO 9167-1 method based on the HPLC analysis of desulphoglucosinolates. Isolation of glucosinolates from used plant material was performed using the method of glucosinolate extraction by desulphation. The following glucosinolates were isolated and quantizated from Nasturtium officinale: methylsulfinylalkyl glucosinolates i.e. glucohesperin, 7-(methylsulfinyl)heptylglucosinolate, glucohirsutin, than gluconasturcin which is arylalkyl glucosinolate, and glucobrasicin and 4-methoxyglucobrasicin which belong indole glucosinolates group. The following compounds were quantizated in Iberis umbellata seeds: glucoiberin, sinigrin, gluconapin, glucoibervirin and glucotropeolin. While from Iberis umbellata plant cultivated using the same seed following compounds were indentified: glucoiberin, sinigrin, glucoibervirin, glucotropeolin and 4-methoxyglucobrasicin. Although present in seeds, gluconapin was not found in cultivated Iberis umbellata plant; on the other hand 4-methoxyglucobrasicin was identified in the plant whie beeing absent in the seeds.