The immortelle is a typically Mediterranean plant which belongs to the genus Helichrysum, family Asteraceae, and comprises about six hundred different species widespread all over the world. In the last few years, the immortelle has become particularly interesting and important for the pharmaceutical, cosmetic, and food industries. It exhibits numerous biological activities, such as anti-inflammatory, antimicrobial, repellent, antiviral, and others. The aim of this study was to determine the chemical profile of immortelle essential oil from the island of Hvar, to isolate and identify volatile compounds from air- dried plant material, and to compare the chemical profile of essential oil and hydrolate obtained in a laboratory and in an industry. The plant material (Helichrysum italicum (Roth) G. Don ssp. italicum) used in this study was collected on the island of Hvar in June. The volatile compounds of immortelle were isolated by hydrodistillation using apparatus according to the European Pharmacopoeia. The chemical analysis of the essential oil and hydrolate was carried out by gas chromatography-mass spectrometry (GC-MS). The major constituents of immortelle essential oil are the sesquiterpene hydrocarbon γ-curcumene (12.16%) and the monoterpene hydrocarbon α-pinene (10.54%), followed by the ester neryl acetate with 7.87%, as well as the diketones italidione I and II. The dominant chemical compounds in both hydrolate samples, obtained in the laboratory and industry, show similarities in their chemical profiles. The main components are the diketone italidione I (12.45% and 7.86%), the monoterpene alcohol nerol (10.38% and 7.81%), and the ketone 4,6- dimethyloctane-3,5-dione (6.99% and 6.81%). Compared to the compounds analyzed in the essential oil, the hydrolates do not contain γ-curcumene, and neryl acetate is present in very small amount, while α- pinene is found only in trace amounts. Based on the conducted research and comparison with previous studies, similarities in the chemical profile of immortelle essential oil can be observed.