MS-DIAL is software developed in 2015 for mass spectrometry data analysis, specifically for the identification and quantification of metabolites and lipids. It is used for analyzing complex biological samples like blood, urine, and tissue, employing GC-MS, LC-MS, and MS/MS techniques. Through spectral deconvolution, the software enables distinguishing overlapping ion signals and accurate compound identification. It integrates with databases like MassBank, facilitating comparison and identification of metabolites. Additionally, MS-DIAL supports both relative and absolute quantification of compounds, allowing precise concentration measurements. Its data visualization, automation of analyses, and flexibility have contributed to its widespread use in biomedical research, metabolomics, and environmental science. Significant software upgrades over the years include open-source code and advanced algorithms, enabling further development and customization. MS-DIAL has become a key tool in research related to diseases like diabetes and cardiovascular conditions, significantly advancing lipidomics and metabolomics analysis.