Global progress has created many problems in protecting and preserving the environment. Soil contamination with toxic substances has increased and become a global problem. Many of the pollutants are carcinogenic and mutagenic and represent a serious problem to human health. Soil pollution with heavy metals is one of the biggest concerns of today. Some types of heavy metals are toxic and deadly, even in small concentrations, while others can cause neurological disorders and other serious illnesses. Therefore, remediation of soils contaminated with heavy metals is very important and nowadays the emphasis is placed on phytoremediation as an economical and environmentally friendly method. In this diploma thesis, the concentration of residual copper, as a heavy metal, in humus in which two plants grew was determined; rocket and garden candytuft. Both plants served as copper hyperaccumulators. Copper content was determined in six differently prepared humus (a total of 12 for both plants): pure humus without additives, humus with the addition of fly ash on which copper was adsorbed, humus with the addition of humus on which copper was adsorbed, humus with the addition of egg shells on which copper was adsorbed, humus with the addition of zeolite on which copper was adsorbed, and the last, humus in which the plants were watered only with an aqueous solution of copper(II) sulphate. Elevated copper content is observed in all humus samples except in control samples. Based on the measured copper content, it can be concluded that the plants still, to a greater or lesser extent, adopted copper from humus to which adsorbents were added to which copper was previously adsorbed as a heavy metal, as well as those watered exclusively with aqueous copper(II ) sulphate.