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undergraduate thesis
Kvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike
završni rad
2021. urn:nbn:hr:167:267395
Perica, Petra Božena
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

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Perica, P. B. (2021). Kvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike : završni rad (Undergraduate thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:267395

Perica, Petra Božena. "Kvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:167:267395

Perica, Petra Božena. "Kvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:167:267395

Perica, P. B. (2021). 'Kvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, accessed 16 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:267395

Perica PB. Kvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2021 [cited 2024 November 16] Available at: https://urn.nsk.hr/urn:nbn:hr:167:267395

P. B. Perica, "Kvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:167:267395

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Metadata
TitleKvalitativno i kvantitativno određivanje vitamina B1, B2 i B6 uporabom HPLC-DAD tehnike : završni rad
Title (english)Qualitative and quantitative determination of vitamins B1, B2 and B6 using HPLC-DAD : bachelor thesis
AuthorPetra Božena Perica
MentorFranko Burčul (mentor)
Committee memberLea Kukoč Modun (predsjednik povjerenstva)
Committee memberIvica Blažević (član povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2021-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Završni rad opisuje razvoj i optimizaciju te vrednovanje metode kvantitativnog i kvalitativnog određivanja vitamina B 1 , B 2 i B 6 u prahu i kapsulama pomoću tekućinske kromatografije visoke djelotvornost spregnute s detektorom s nizom dioda. U istraživanju je korišten radni standard vitamina B kompleksa od kojeg su pripravljene matične otopine korištene za optimizaciju metode određivanja vitamina B 1 , B 2 i B 6 pomoću tekućinskog kromatografa s DAD spektrom kao detektorom. Kao otapalo izbora za B kompleks izabrana je smjesa acetonitrila i ultračiste vode + 0,3% fosforne kiseline u omjeru 10:90. Stacionarna faza je kolona s C18 punilom. Ispitane značajke vrednovanja koje uključuju specifičnost, linearnost, točnost, preciznost, granicu određivanja i dokazivanja i robusnost dale su zadovoljavajuće vrijednosti. Tekućinska kromatografija visoke djelotvornosti spregnuta s detektorom s nizom dioda je prikladna tehnika za kvantitativno i kvalitativno određivanje vitamina B 1 , B 2 i B 6 u prahu i kapsulama. Razvijena metoda je vrednovana čime je osigurana njena prikladnost za određivanje vitamina B 1 , B 2 i B 6 .
Abstract (english)
The bachelor thesis describes development, optimization and validation of the method for quantitative and qualitative determination of vitamina B 1 , B 2 i B 6 in powder and capsules by high performance liquid chromatography coupled to diode array detector. The study used a standard of vitamin B complex from which main solutions were used to optimize a method of determination vitamins B 1 , B 2 i B 6 using a liquid cromatograph with DAD spectrometry as detector. The solvent of choice for B complex was a mixture of acetonitrile and ultra-pure water + 0.3 % phosphoric acid in a ratio of 10:90. Stationary phase was column with C18 filler. Tested validation parameters including specificity, linearity, accuracy, precision, limit of quantification and detection, stability and robustness resulted with adequate values. High performance liquid chromatography coupled to a diode array detector is a suitable technique for the quantitative and qualitative determination of vitamin B 1 , B 2 i B 6 in powder and capsules. The method has been validated to ensure its suitability for the determination of vitamin B 1 , B 2 i B 6 .
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:267395
Study programmeTitle: Chemistry
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2021-12-15 11:26:36