prikaz prve stranice dokumenta Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem
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master's thesis
Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem
diplomski rad
2016. urn:nbn:hr:167:388437
Leskur, Željana
University of Split
Faculty of Chemistry and Technology
Division of Chemistry
University of Split
School of Medicine

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Leskur, Ž. (2016). Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:388437

Leskur, Željana. "Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:167:388437

Leskur, Željana. "Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:167:388437

Leskur, Ž. (2016). 'Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 18 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:388437

Leskur Ž. Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2016 [cited 2024 November 18] Available at: https://urn.nsk.hr/urn:nbn:hr:167:388437

Ž. Leskur, "Razvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:167:388437

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TitleRazvoj i vrednovanje spektrofotometrijske metode određivanja tiopronina u farmaceutskim pripravcima slijednom analizom injektiranjem : diplomski rad
Title (english)DEVELOPMENT AND VALIDATION OF SEQUENTIAL INJECTION SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TIOPRONIN IN PHARMACEUTICAL FORMULATIONS : master thesis
AuthorŽeljana Leskur
MentorLea Kukoč Modun (mentor)
Committee memberZoran Grubač (predsjednik povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2016-10-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
Korištenjem slijedne analize injektiranjem uz spektrofotometrijski detektor optimizirana je i vrednovana nova metoda određivanja tiopronina (N-2-merkaptopropionil-glicina, MPG) u farmaceutskim pripravcima. Metoda se temelji na redoks-reakciji između bis neokuproin bakrovog(II) kompleksa i tiopronina pri čemu nastaje bis neokuproin bakrov(I) obojeni kompleks, ţuto-narančaste boje. Nastali kompleks pokazuje apsorpcijski maksimum pri λ = 458 nm. Univarijantnom metodom odabrani su optimalni parametri protočnog sustava te je temeljem toga konstruirana krivulja umjeravanja čijom je regresijskom analizom utvrđena jednadţba pravca: y = 3413,1x + 0,0007. Postignuta je linearnost u području koncentracija analita od 6×10⁻⁷ mol L⁻¹ do 8×10⁻⁵ mol L⁻¹. Određene su granica dokazivanja (2×10⁻⁷ mol L⁻¹) i granica određivanja (6×10⁻⁷ mol L⁻¹). Provedeno je vrednovanje metode te je provjereno prenošenje signala, ponovljivost signala, točnost i iskoristivost metode. Osim acetilsalicilne kiseline i visokih koncentracija vinske kiseline i laktoze, tvari koje se uobičajeno nalaze u sastavu farmaceutskih pripravaka ne interferiraju. Predloţena metoda uspješno je primijenjena kod određivanja tiopronina u farmaceutskom pripravku što je uspoređeno s rezultatima dobivenim farmakopejskom metodom.
Abstract (english)
By using sequetial injection analysis with spectrophotometric detector a novel procedure was optimized and validated for the determination of tiopronin (N-2-Mercaptopropionyl-glycine, MPG) in pharmaceutical formulations. The method is based on a redox reaction in which the reduction of copper(II) - neocuproine by MPG occurs, with formation of colored, yellow-orange complex of copper(I) - neocuproine. The resulting complex exhibits an absorption maximum at λ = 458 nm. Univariate method was used to select optimal parameters of the flow system. As a result the calibration curve was plotted and using the regression analysis the equation: y = 3413,1x + 0,0007 was determined. The linearity in the concentration range of the analyte from 6×10⁻⁷ mol L⁻¹ to 8×10⁻⁵ mol L⁻¹ was achieved with that. The calculated limit of detection is 2×10⁻⁷ mol L⁻¹ and the limit of quantification is 6×10⁻⁷ mol L⁻¹. Validation of the method included evaluating carryover, signal repeatability and recovery (accuracy and efficiency of the method). Besides aspirin, and high concentrations of lactose and tartaric acid, substances that are normally present in the composition of the pharmaceutical formulations do not interfere. The proposed method was successfully applied for the determination of MPG in its pharmaceutical formulation, which is compared to the results obtained by the pharmacopoeia method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:388437
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-11-22 10:23:12