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master's thesis
Glukozinolatni profil biljke Alliaria petiolata
diplomski rad
2023. urn:nbn:hr:167:547248
Rašić, Andreja
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

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Rašić, A. (2023). Glukozinolatni profil biljke Alliaria petiolata : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:547248

Rašić, Andreja. "Glukozinolatni profil biljke Alliaria petiolata : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:547248

Rašić, Andreja. "Glukozinolatni profil biljke Alliaria petiolata : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:547248

Rašić, A. (2023). 'Glukozinolatni profil biljke Alliaria petiolata : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 16 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:547248

Rašić A. Glukozinolatni profil biljke Alliaria petiolata : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2023 [cited 2024 November 16] Available at: https://urn.nsk.hr/urn:nbn:hr:167:547248

A. Rašić, "Glukozinolatni profil biljke Alliaria petiolata : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:167:547248

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Metadata
TitleGlukozinolatni profil biljke Alliaria petiolata : diplomski rad
Title (english)Glucosinolates profile of the plant Alliaria petiolata : diploma thesis
AuthorAndreja Rašić
MentorIvica Blažević (mentor)
Committee memberSandra Svilović (predsjednik povjerenstva)
Committee memberAzra Đulović (član povjerenstva)
Committee memberIvica Blažević (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2023-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U ovom radu izolirani su i analizirani glukozinolati ljekovite češnjače (Alliaria petiolata). Cilj rada bio je istražiti glukozinolatni profil u različitim biljnim dijelovima. Analiza je provedena prema službenoj ISO 9167-1 metodi u kojoj se analiza provodi preko desulfoglukozinolata korištenjem UHLPC-MS/MS instrumenta. Također UHLPC-MS/MS tehnika je korištena za analizu intaktnih glukozinolata prisutnih u listu. Analizirani su i razgradni produkti glukozinolata nakon autolize biljnog materijala korištenjem GC-MS. UHLPC-MS analiza desulfoglukozinolata pokazala je da su u svim biljnim dijelovima (korijen, stabljika, list, cvijet i komuška) prisutni sinigrin (1,5840 –97,5028 µmol/g) i glukotropeolin (0,0373–1,3406 µmol/g). U komuški, cvijetu i stabljici pronađen je i 4-hidroksiglukobrasicin (0,0078–1,4868 µmol/g), dok je u korijenu pronađen i 4-metoksiglukobrasicin (0,16501µmol/g) i glukosinabin u tragovima. UHPLC-MS tehnika korištena je i za analizu metanol-vodenog ekstrakta lista. Identificirana su 3 spoja, sinigrin, izoviteksin-6''-O-β-D-glukopiranozid i aliarinozid koji je pronađen samo u biljci Alliaria petiolata. GC-MS tehnika korištena je za analizu diklormetanskog ekstrakta biljnog materijala nakon autolize. Identficirano je 12 različitih spojeva među kojima nisu pronađeni razgradni produkti glukozinolata.
Abstract (english)
In this work, glucosinolates from Alliaria petiolata were isolated and analyzed. The aim of the work was to investigate the profile of glucosinolates in different parts of the plant. The analysis was made according to the official ISO 9167-1 method based on the UHPLC-MS/MS analysis of desulfoglucosinolate. The UHLPC- MS/MS technique was also used for the analysis of intact glucosinolates present in the leaf. GC-MS technique was used for analyze of glucosinolate degradation products after autolysis. Sinigrin (1.5840-97.5028 mol/g) and glucotropeolin (0.0373-1.3406 mol/g) were found in all plant parts of A. petiolata using UHLPC-MS/MS analysis of desulfoglucosinolates. 4-Hydroxyglucobrassicin (0.0078-1.4868 mol/g) was found in the siliquae, flower and stem, whereas 4-methoxyglucobrassicin (0.16501 mol/g) and glucosinabin (in traces) were found in the root. The UHPLC-MS technique was also used for the analysis of the methanol-water leaf extract. Three (3) compounds were identified sinigrin, isovitexin-6''-O-β-D-glucopyranoside and alliarinoside, which was found only in Alliaria petiolata plant. The GC-MS technique was used to analyze the dichloromethane extract of the plant sample after autolysis. There were twelve (12) distinct substances found, but none of them were glucosinolate's breakdown products.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:547248
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-12-01 09:52:34