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master's thesis
Biotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama
diplomski rad
2023. urn:nbn:hr:167:520220
Kirić, Marija
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

Cite this document

Kirić, M. (2023). Biotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:520220

Kirić, Marija. "Biotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:520220

Kirić, Marija. "Biotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:520220

Kirić, M. (2023). 'Biotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 27 April 2024, https://urn.nsk.hr/urn:nbn:hr:167:520220

Kirić M. Biotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2023 [cited 2024 April 27] Available at: https://urn.nsk.hr/urn:nbn:hr:167:520220

M. Kirić, "Biotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:167:520220

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Metadata
TitleBiotransformacija eufoboetirana A s gljivicom Sordaria tormento-alba s ciljem potrage za novim bioaktivnim molekulama : diplomski rad
Title (english)Biotransformation of euphoboetirane A by Sordaria tormento-alba aimed at the search for new bioactive molecules : diploma thesis
AuthorMarija Kirić
MentorAni Radonić (mentor)
Committee memberBranka Andričić (predsjednik povjerenstva)
Committee memberAni Radonić (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2023-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Latirani, koji se često nalaze u biljkama roda Euphorbia, pripadaju skupini biološki aktivnih policikličkih diterpenoida. Ova grupa spojeva i njihovi sintetski derivati dobili su značajnu pozornost zahvaljujući svojim biološkim aktivnostima i kliničkom potencijalu protiv različitih bolesti kao što je višestruka otpornost na lijekove (MDR), protuupalno djelovanje, citotoksično djelovanje protiv staničnih linija raka, antivirusna svojstva i njihova sposobnost induciranja proliferacije ili diferencijacije u neurone neuralnih progenitorskih stanica. U ovom radu provedena je izolacija, pročišćavanje i biotransformacija eufoboetirana A (4) pomoću endofitne gljivice Sordaria tomento-alba. Cijele stanice kulture ove gljivice korištene su za biotransformaciju početnog supstrata (4) te su dobivena dva produkta biotransformacije od interesa, koji su odvojeni i pročišćeni kolonskom kromatografijom i HPLC-om. Strukture dobivenih produkata biotransformacije razjašnjene su na temelju opsežnih NMR i MS analiza podataka. Identificirani su kao Sordarianona (23) i 3,5,15-triacetoksi-12-hidroksilatira-6(17),13(20)-dien-14-on (24). Ovo istraživanje pokazuje biotransformacijsku sposobnost endofitne gljivice Sordaria tomento-alba da vrši intramolekularne cikloadicije, hidroksilacije i izomerizacije dvostrukih ugljik-ugljik veza. Ove reakcije mogu dovesti do derivata latirana s pojačanim biološkim aktivnostima, što bi moglo pomoći u rasvjetljavanju strukturnih značajki po kojima ti spojevi pokazuju potencijal za liječenje neurodegenerativnih poremećaja.
Abstract (english)
Lathyranes, frequently found in plants of the genus Euphorbia, belong to the group of biologically active polycyclic diterpenoids. This family of compounds and their synthetic derivatives have received considerable attention owing to their interesting biological activities and clinical potential against different diseases like multi-drug resistance (MDR) reversal, anti-inflammatory activity, cytotoxic activity against cancer cell lines, antiviral properties and their capability to induce proliferation or differentiation into neurons of neural progenitor cells. In this work, it has been carried out the isolation, purification and biotransformation of euphoboetirane A (4) by the endophytic fungus Sordaria tomento-alba. Whole cell culture of this fungus was used to biotransform the initial substrate (4) in two biotransformation products of interest, which were separated and purified by the column chromatography and HPLC. The structures of obtained biotransformation products were elucidated on the basis of extensive NMR and MS data analyses. They were identified as Sordarianona (23) and 3,5,15-triacetoxy-12-hydroxylathyra-6(17),13(20)-dien-14-on (24). This research shows biotransformation ability of endophytic fungus Sordaria tomento-alba to perform intramolecular cycloadditions, hydroxylations and isomerisations of carbon-carbon double bonds. These reactions may lead to lathyrane derivatives with enhanced biological activities, which could help to clarify the structural features by which these compounds show potential for the treatment of neurodegenerative disorders.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:520220
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-12-01 09:49:37