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master's thesis
Dokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima
diplomski rad
2017. urn:nbn:hr:167:781481
Vlašić, Hrvoje
University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

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Vlašić, H. (2017). Dokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:781481

Vlašić, Hrvoje. "Dokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:781481

Vlašić, Hrvoje. "Dokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:781481

Vlašić, H. (2017). 'Dokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 16 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:781481

Vlašić H. Dokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2024 December 16] Available at: https://urn.nsk.hr/urn:nbn:hr:167:781481

H. Vlašić, "Dokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:781481

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Metadata
TitleDokazivanje THC-a u medicinskim pripravcima i u biološkim uzorcima : diplomski rad
Title (english)Determination of THC in medical cannabis and biological samples : diploma thesis
AuthorHrvoje Vlašić
MentorDavorka Sutlović (mentor)
Committee memberDavorka Sutlović (predsjednik povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2017-05-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
Ciljevi istraživanja: Kvalitativno dokazati prisutnost THC-a i ostalih kanabinoida u medicinskim pripravcima i u biološkim uzorcima primjenom GC-MS metode, usporediti rezultate analize dobivene nakon pripreme uzoraka različitim ekstrakcijskim metodama (ekstrakcija čvrstom fazom, SPE, i ekstrakcija tekuće-tekuće, LLE) te utvrditi koja je metoda prikladnija za pripremu uzoraka pri analizi kanabinoida. Materijali i metode: Tri medicinska pripravka koji sadrže THC otopljena su i razrijeđena u kloroformu te analizirana GC-MS instrumentalnom metodom kako bi se utvrdio njihov sastav. Zatim su, koristeći te pripravke, pripravljena 24 biološka uzorka, od toga 12 uzoraka s mokraćom i 12 uzoraka sa serumom. Uzorci su podijeljeni u dvije istovjetne skupine i svaka je za analizu pripremljena različitom ekstrakcijskom metodom, ekstrakcijom čvrstom fazom (SPE) ili ekstrakcijom tekuće-tekuće (LLE). Uzorci su otopljeni u malom volumenu kloroforma i analizirani na GC-MS uređaju metodom koja omogućava istovremeno snimanje ukupnog ionskog kromatograma (TIC) u području od 40 – 600 m/z i snimanje samo odabranih iona (SIM).
Abstract (english)
Objectives: Qualitative determination of THC and other cannabinoids in medical preparations and biological samples using GC-MS method, comparison of the results obtained after sample preparation with different extraction methods (Solid phase extraction, SPE, and Liquid-liquid extraction, LLE) and defining the more appropriate extraction method for sample preparation in cannabinoids analysis. Materials and methods: Three medical preparations which contain THC were dissolved and diluted in chloroform and analyzed with GCMS instrumental method in order to determine their composition. Then, that preparations were used to make 24 biological samples with different concentrations of cannabinoids, 12 samples of urine and 12 samples of serum. Samples were divided into two identical groups and each group was prepared for analysis using different extraction method, solid-phase extraction or liquid-liquid extraction. Samples were dissolved in a small volume of chloroform and analyzed with GC-MS method using mode that simultaneously records total ion chromatogram (TIC) in the area of 40-600 m/z and single ion monitoring (SIM) scanning mode.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:781481
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar/ Key words are revised by the librarian
Created on2017-07-06 11:19:54