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undergraduate thesis
Pregled metoda u staničnoj genetici
završni rad
2020. urn:nbn:hr:167:946270
Velić, Marina
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

Cite this document

Velić, M. (2020). Pregled metoda u staničnoj genetici : završni rad (Undergraduate thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:946270

Velić, Marina. "Pregled metoda u staničnoj genetici : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:167:946270

Velić, Marina. "Pregled metoda u staničnoj genetici : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:167:946270

Velić, M. (2020). 'Pregled metoda u staničnoj genetici : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, accessed 27 October 2024, https://urn.nsk.hr/urn:nbn:hr:167:946270

Velić M. Pregled metoda u staničnoj genetici : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2020 [cited 2024 October 27] Available at: https://urn.nsk.hr/urn:nbn:hr:167:946270

M. Velić, "Pregled metoda u staničnoj genetici : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:167:946270

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Metadata
TitlePregled metoda u staničnoj genetici : završni rad
Title (english)A review of methods in cell genetics : bachelor thesis
AuthorMarina Velić
MentorIvana Carev (mentor)
Committee memberMila Radan (predsjednik povjerenstva)
Committee memberMladen Miloš (član povjerenstva)
Committee memberIvana Carev (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2020-09-21, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Genetika je mlada znanost koja se bavi proučavanjem zakonitosti nasljeđivanja, sličnosti i različitosti živih organizama. Genetika proučava strukturu i funkciju gena, nasljeđivanje gena, promjene koje se događaju u organizmima i mnoge uzročnike bolesti. Citogenetika je grana genetike koja proučava stanične strukture koje su odgovorne za nasljeđivanje. Tako ona istražuje građu i broj kromosoma, smještaj gena i mutacije. Cilj ovog rada bio je opisati metode u staničnoj genetici, citogenetici. U radu su detaljno opisane metode protočne citometrije i metoda fluorescnetne in situ hibridizacije (FISH). Protočna citometrija je metoda koja omogućava brzu, osjetljivu i točnu analizu velikog broja staničnih komponenti. Ona se koristi za izolaciju specifičnih stanica ili staničnih jezgri iz smjese stanica na osnovu različitog raspršivanja svjetlosti, odnosno fluorescencije. Upravo fluorescencija razdvaja stanice koje su obilježene različitim fluorescentnim reagensima. Fluorescentna in situ hibridizacija je tehnika koja uz pomoć nukleinske sonde, odnosno probe, uočava određenu sekvencu u kromosomu. Sonda predstavlja fluorescentno označenu molekulu DNA koja je komplementarna sekvenci genoma kojeg treba otkriti. Tako je ovom tehnikom moguće čitave kromosome predstaviti u bojama. Metoda FISH upotrebljava se za identificiranje gena, dijelova gena, kromosoma i za otkrivanje pojedinih promjena u kromosomima.
Abstract (english)
Genetics is a young science that deals with the study of the laws of inheritance, similarities and differences of living organisms. Genetics studies the structure and function of genes, gene inheritance, changes that occur in organisms, and many pathogens. Cytogenetics is a branch of genetics that studies cellular structures responsible for inheritance. Thus, she investigates the structure and number of chromosomes, gene placement and mutations. The aim of this work was to describe methods in cellular genetics, cytogenetics. This work describes the flow cytometry method and the fluorescent in situ hybridization (FISH) method. Flow cytometry is a technique that allows fast, sensitive and accurate analysis of a large number of cellular components. It is used to isolate specific cells or cell nuclei from a mixture of cells based on different light scattering or fluorescence. Fluorescent in situ hybridization is a technique that uses a nucleic to detect a specific sequence in a chromosome. The probe is a fluorescently labeled DNA molecule that is complementary to the sequence of the genome to be detected. Thus, with this technique, it is possible to present entire chromosomes in colors. The FISH method is used to identify genes, parts of genes, chromosomes and to detect individual changes in chromosomes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:946270
Study programmeTitle: Food technology - undergraduate - university
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik (baccalaureus) inženjer prehrambene tehnologije (sveučilišni prvostupnik (baccalaureus) inženjer prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-12-17 13:15:46