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master's thesis
Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor
diplomski rad
2017. urn:nbn:hr:167:909602
Rogošić, Ante
University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

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Rogošić, A. (2017). Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:909602

Rogošić, Ante. "Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:909602

Rogošić, Ante. "Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:909602

Rogošić, A. (2017). 'Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:909602

Rogošić A. Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:167:909602

A. Rogošić, "Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:909602

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TitleRazvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad
Title (english)Sequential injection of glutathione in pharmaceutical formulations using spectrophotometric detector. Development and validation of the method. : diploma thesis
AuthorAnte Rogošić
MentorLea Kukoč Modun (mentor)
Committee memberFranko Burčul (predsjednik povjerenstva)
Committee memberIvana Škugor Rončević (član povjerenstva)
Committee memberLea Kukoč Modun (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2017-10-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
U ovom je radu optimizirana i vrednovana metoda odreĎivanja glutationa slijednom analizom injektiranjem uz spektrofotometrijski detektor. Metoda se temelji na redoks-reakciji izmeĎu bakar(II)- neokuproin kompleksa i glutationa (GSH). Glutation ima ulogu reducensa te kao produkti nastaju oksidirani oblik glutationa (GSSG) i bakar(I)-neokuproin kompleks koji postiţe apsorpcijski maksimum pri λ = 458 nm. Optimizacijom parametara protočnog sustava obuhvaćeni su redoslijed injektiranja reagensa i uzorka, brzina protoka osnovne otopine, volumeni injektiranja analita i uzorka, duljina reakcijske uzvojnice te volumen petlje zadrţavanja. Tako optimiziranom metodom ostvareno je linerano dinamičko područje (LDP) u koncentracijskom rasponu od 6,00 × 10 ─7 mol L ─1 do 8,00 × 10 ─5 mol L ─1 uz pripadajuću jednadţbu pravca y = 3270 x – 0,0010 i koeficijent linearne regresije R 2 = 0,9983.Izmjerene su vrijednosti posluţile kao temelj za računanje granice dokazivanja (LOD = 2,20 × 10 ─7 mol L ─1 ) i granice odreĎivanja (LOQ = 6,00 × 10 ─7 mol L ─1 ). U odreĎivanju analita predloţenom metodom interferirali su: limunska kiselina, vinska kiselina, acetilsalicilna kiselina te natrijev citrat. Temeljem 15 uzastopnih injektiranja standardne otopine uzorka utvrĎena je ponovljivost metode uz relativno standardno odstupanje, RSD = 3,80 %. Mjerenjem smjesa otopina realnog uzorka i standardne otopine glutationa različitih koncentracija vrednovana je točnost metode. UsporeĎeni su rezultati odreĎivanja glutationa u realnom uzorku predloţenom metodom i metodom preporučenom farmakopejom. Slaganje rezultata (52,00 mg i 50,86 mg) upućuje na točnost metode.
Abstract (english)
A method for determination of glutathione using sequential injection analysis had been optimized and validated. The method is based on a redox reaction between glutathione and Cu(II)-neocuproine complex where glutathione is a reducens. Reaction products are oxidised form of glutathione (GSSG) and Cu(I)-neocuproine complex which reaches its absorption maximum at λ = 458 nm. Optimized parameters of flow system include sequence of injection of sample and reagent, rate of flow of carrier solution, volumes of injection of sample and reagent, volume of holding coil and lenght of reaction coil. Optimized method parameters were used to plot the callibration curve. The following equation had been determined: y = 3270 x – 0,0010. Linearity is achieved at analyte concentrations ranging from 6,00 × 10 ─7 mol L ─1 to 8,00 × 10 ─5 mol L ─1 . Limit of detection is LOD = 2,20 × 10 ─7 mol L ─1 while the limit of quantification is 6,00 × 10 ─7 mol L ─1 . Following compounds interfere: acetylsalicilyc acid, tartaric acid, citric acid and natrium citrate. Repeatability was confirmed based on results of 15 sequential injections of standard solution of glutathione. Relative standard deviation was 3,80 %. Method accuracy was validated by determining concentrations of mixed solutions composed of glutathione standard solution and glutathione real sample solution. Results of the developed method were compared with results of pharmacopoeial method. Similar results (52,00 g and 50,86 g) indicate method accuracy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:909602
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-08-22 10:01:00