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master's thesis
Kvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS
diplomski rad
2024. urn:nbn:hr:167:534353
Delaš, Bruna
University of Split
Faculty of Chemistry and Technology
Division of Chemistry
Department of Analytical Chemistry

Cite this document

Delaš, B. (2024). Kvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:534353

Delaš, Bruna. "Kvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:167:534353

Delaš, Bruna. "Kvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:167:534353

Delaš, B. (2024). 'Kvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 23 January 2025, https://urn.nsk.hr/urn:nbn:hr:167:534353

Delaš B. Kvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2024 [cited 2025 January 23] Available at: https://urn.nsk.hr/urn:nbn:hr:167:534353

B. Delaš, "Kvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:167:534353

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Metadata
TitleKvalitativno i kvantitativno određivanje ibuprofena i karbendazima pomoću UHPLC-DAD-MS/MS : diplomski rad
Title (english)Qualitative and quantitative determination of ibuprofen and carbendazim using UHPLC-DAD-MS/MS : diploma thesis
AuthorBruna Delaš
MentorFranko Burčul (mentor)
Committee memberIvica Blažević (predsjednik povjerenstva)
Committee memberIvona Nuić (član povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
(Department of Analytical Chemistry)
Split
Defense date and country2024-10-04, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Ovaj rad pruža teorijski uvid u biološki aktivne tvari ibuprofen i karbendazim, te način njihovog dospijevanja u okoliš. Također, opisani su osnovni principi kromatografskih tehnika s naglaskom na UHPLC-DAD-MS/MS te razvoj instrumentalne metode. Kao otapalo za pripravu standardnih otopina ibuprofena i karbendazima odabran je metanol. Eksperimentalno je odabrana kolona te sastav pokretne faze za tekućinsku kromatografiju. Uz to optimiziran je protok, gradijent pokretne faze, temperatura kolone i volumen injektiranja. Na trostrukom četveropolnom spektrometru masa određene su vrijednosti m/z iona prekursora i produkata, odnosno prijelazi karakteristični za svaki analit te pripadajuće vrijednosti energija sraza. Optimiziran je i tlak inertnog plina koji omogućuju disocijaciju molekulskog iona kao i ostali parametri spektrometrije masa. Metoda je u konačnici vrednovana određivanjem: specifičnosti, linearnog dijela krivulje umjeravanja, granica dokazivanja i određivanja, točnosti, preciznosti, prikladnosti sustava, robusnosti i stabilnosti otopine analita. Naposljetku, na temelju dobivenih rezultata, donesen je zaključak o prikladnosti metode za korištenje pri analizi realnih uzoraka.
Abstract (english)
This paper provides a theoretical insight into the biologically active substances ibuprofen and carbendazim and how they enter the environment. Additionally, the basic principles of the chromatography techniques in particular UHPLC-DAD-MS/MS are described, as well as the development of the instrumental method. To prepare standard solutions of ibuprofen and carbendazim, methanol was selected as a solvent. The column and the composition of the mobile phase for the liquid chromatograph were selected experimentally. Additionally, the flow, mobile phase gradient, column temperature and injection volume were optimized too. On the triple quadrupole mass spectrometer, the m/z values of precursor ions and product ions, characteristic transitions for each analyte, and the corresponding collision energy values were determined. Inert gas pressure that enables the dissociation of the molecular ions as well as other mass spectrometry parameters were optimized too. The method was validated by determining specificity, the linear part of the calibration curve, limits of detection and quantification, accuracy, precision, system suitability, robustness, and analyte solution stability. Finally, based on the obtained results, a conclusion about the suitability of the method for use in real samples analysis was made.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:534353
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-01-23 09:31:38