Sea fennel (Crithmum maritimum L.) is one of the best known halophytic species of the Adriatic coast, which plays an important role in the food, chemical industry and medicine due to its rich chemical profile and adaptability. The most important constituents of sea fennel include volatile components of essential oils, fatty acids, phenolics, minerals, vitamins, carotenoids, and others. The aim of this study was to identify and quantify tocopherols (α-, β- and γ-) present in sea fennel samples using direct acetone extraction and saponification methods followed by high performance liquid chromatography (HPLC). The study was conducted on 13 different samples (10 leaf samples and 3 flower samples) from 10 locations along the Adriatic coast. After analyzing the results, it was found that the highest concentration of total tocopherols was obtained from the leaves harvested on Pelješac and the flowers harvested on Pag. α-tocopherol was the dominating chemical species in all samples with concentrations ranging from 3.87 µg/mL in the sample from Šibenik to 21.57 µg/mL in the sample from Pelješac. The sea fennel sample from Pelješac had the highest content of β-tocopherol, while the highest content of γ-tocopherol was found in the sample collected in Šibenik.