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master's thesis
Određivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor
diplomski rad
2022. urn:nbn:hr:167:738380
Dugeč, Josipa
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

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Dugeč, J. (2022). Određivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:738380

Dugeč, Josipa. "Određivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:167:738380

Dugeč, Josipa. "Određivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2022. https://urn.nsk.hr/urn:nbn:hr:167:738380

Dugeč, J. (2022). 'Određivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 25 May 2023, https://urn.nsk.hr/urn:nbn:hr:167:738380

Dugeč J. Određivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2022 [cited 2023 May 25] Available at: https://urn.nsk.hr/urn:nbn:hr:167:738380

J. Dugeč, "Određivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:167:738380

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Metadata
TitleOdređivanje fenicilamina u farmaceutskim pripravcima kinetičkom metodom analize uz spektrofotometrijski detektor : diplomski rad
Title (english)Determination of penicillamine in pharmaceutical formulations using kinetic method with spectrofhotometric detector : master thesis
AuthorJosipa Dugeč
MentorLea Kukoč Modun (mentor)
Committee memberFranko Burčul (predsjednik povjerenstva)
Committee memberMario Nikola Mužek (član povjerenstva)
Committee memberLea Kukoč Modun (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2022-07-19, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Korištenjem kinetičke spektrofotometrije razvijena je i vrednovana nova metoda određivanja penicilamina (PEN) u farmaceutskim pripravcima. Metoda se temelji na redoks reakciji između bis batokuproin bakrov(II) kompleksa i penicilamina pri čemu nastaje stabilan kompleks narančaste boje, bis batokuproin bakrov(I) kompleks. Nastali kompleks postiže apsorpcijski maksimum pri valnoj duljini od 483 nm. Optimizirani su parametri koji utječu na kemijsku reakciju. Kod izrade krivulja umjeravanja korištene su metoda početne brzine i metoda odabranog vremena. Krivulja umjeravanja metodom početne brzine linearna je u području koncentracija od 6,0 × 10 −7 do 4,0 × 10 −5 mol L −1 , s granicom dokazivanja od 1,8 × 10 −7 mol L –1 . Nagib krivulje umjeravanja dobiven metodom početne brzine (1,0448) potvrđuje reakciju pseudoprvog reda. Krivulja umjeravanja metodom odabranog vremena (prva minuta) linearna je u području koncentracija od 2,0 × 10 −7 do 4,0 × 10 −5 mol L −1 , s granicom dokazivanja od 6,0 × 10 −8 mol L –1 . Predložena metoda uspješno je primijenjena kod određivanja penicilamina u komercijalnim farmaceutskim pripravcima što je uspoređeno s rezultatima dobivenim preporučenom metodom po farmakopeji.
Abstract (english)
Kinetic spectrophotometry was employed for development and validation of new method for determination of penicillamine (PEN) in pharmaceutical formulation. The method is based on a redox reaction in which the reduction of copper(II) – neocuproine by penicillamine occurs, with formation of stable coloured, yellow-orange complex of copper(I) – neocuproine. The resulting complex exhibits an absorption maximum at λ = 483 nm. The parameters of chemical reaction have been optimized. The initial rate and fixed time methods were utilized for constructing the calibration graphs. The graph for initial rate method was linear in concentration range from 6,0 × 10 −7 do 4,0 × 10 −5 mol L −1 with the limit of detection 1,8 × 10 −7 mol L –1 . The slope of the initial method calibration curve (1.0448) confirmed the first order reaction. The graph for fixed time method (1 min) was linear in concentration range from 2,0 × 10 −7 do 4,0 × 10 −5 mol L −1 with the limit of detection 6,0 × 10 −8 mol L –1 . No interferences were observed from the excipients that are commonly present in the pharmaceutical formulations. The proposed method was successfully applied for the determination of PEN in its commercial pharmaceutical formulation, which is compared to the results obtained by the recommended method in Pharmacopoeia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:738380
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-05 07:26:11