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master's thesis
Polimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata
diplomski rad
2016. urn:nbn:hr:167:704423
Bebić, Sara
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

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Bebić, S. (2016). Polimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:704423

Bebić, Sara. "Polimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:704423

Bebić, Sara. "Polimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:704423

Bebić, S. (2016). 'Polimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 08 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:704423

Bebić S. Polimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2016 [cited 2024 December 08] Available at: https://urn.nsk.hr/urn:nbn:hr:167:704423

S. Bebić, "Polimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:167:704423

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Metadata
TitlePolimorfizmi gena NR3C1, NR3C2 i FKBP5 u populaciji hrvatskih maturanata : diplomski rad
Title (english)Polymorphisms of NR3C1, NR3C2 and FKBP5 genes in the Croatian secondary school students : diploma thesis
AuthorSara Bebić
MentorMladen Miloš (mentor)
Committee memberIvica Blažević (predsjednik povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
Committee memberIrena Drmić Hofman (član povjerenstva)
Committee memberMladen Miloš (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2016-10-25, Croatia
Scientific / art field,
discipline and subdiscipline		INTERDISCIPLINARY AREAS OF KNOWLEDGE
Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Abstract
U ovom istraživanju analizirana je učestalost alela i genotipova polimorfizma pojedinačnih nukleotida (SNP) i to: rs5522 gena NR3C2, rs6189 i rs6190 gena NR3C1 te rs1360780 gena FKBP5. Iz uzorka sline prikupljenih od 400 maturanata iz 26 gimnazija i strukovnih škola iz 4 najveća hrvatska grada: Zagreba, Splita, Rijeke i Osijeka, izolirana je DNA, i amplificirana metodom PCR u realnom vremenu. Nakon provedene genotipizacije za pojedinačne SNP-ove izračunata su odstupanja od Hardy-Weinbergovog zakona ravnoteže (HWE), za svaki polimorfizam, za svaku skupinu ispitanika iz četiri hrvatska grada te za sva tri SNP-a u cjelokupnom uzorku svih ispitanika. Analiza dobivenih rezultata pokazala je da postoji statistička značajna razlika u broju uočenih i očekivanih genotipova u ispitanika s područja Zagreba (P=0,00437) za polimorfizam rs5522, iz Rijeke (P=0,00012) za dvojni polimorfizam rs6189/rs6190 te Osijeka (P=0,0186) za polimorfizam rs1360780, dok u ostalim gradovima nije uočeno odstupanje od HWE ravnoteže. Analizom cjelokupnog broja ispitanika iz Hrvatske (N=400), uočeno je odstupanje za polimorfizam rs1360780 (P=0,025). Dobiveni rezultati potvrđuju da bi se polimorfizam rs1360780 mogao dobro iskoristiti u populacijskim studijama te da bi povećanjem broja ispitanika, najvjerojatnije, nestalo i odstupanje od HWE pa bi rezultati bili sličniji nekim drugim populacijskim istraživanjima.
Abstract (english)
In this study, we analyzed the genotyping of single nucleotide polymorphism (SNP), as follows: rs5522 from the gene NR3C2, rs6189 and rs6190 from the gene NR3C1 and rs1360780 from the gene FKBP5. DNA was isolated from saliva samples collected from 400 Croatian upper high school seniors from 26 high schools from four largest cities in Croatia: Zagreb, Split, Rijeka and Osijek. Amplification was performed by real time-PCR. After the conducted genotyping we obtained alleles and genotypes for individual SNPs and we calculated deviations from Hardy-Weinberg law of equilibrium (HWE), for each polymorphism, for each group of subjects and for all three SNPs in the overall sample. Statistically significant difference in the number of observed and expected genotypes was found in the population sample from Zagreb (P=0,00437) for rs5522, from Rijeka (P=0,00012) for a double polymorphism rs6189/rs6190 and Osijek (P=0,01861) for rs1360780p, while in other cities deviations from HWE were not observed. In the analysis of the overall sample from Croatia (N= 400), deviation from HWE was observed for rs1360780 polymorphism (P=0,025). The results suggest that rs1360780 polymorphism could be useful in population studies and, most likely, by increasing the number of subjects.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:704423
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Organic Chemistry and Biochemistry
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar/ Key words are revised by the librarian
Created on2017-07-13 08:05:39