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master's thesis
Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom
diplomski rad
Šuman, Frane (2017)
University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

Cite this document

Šuman, F. (2017). Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad (Master's thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:697922

Šuman, Frane. "Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:697922

Šuman, Frane. "Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:697922

Šuman, F. (2017). 'Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 30 June 2020, https://urn.nsk.hr/urn:nbn:hr:167:697922

Šuman F. Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2020 June 30] Available at: https://urn.nsk.hr/urn:nbn:hr:167:697922

F. Šuman, "Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:697922

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Metadata
TitleCitotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad
Title (english)Cytotoxic activity of oleuropein extracted from wild olive leaf in various human cancer cells lines measured by MTT method : diploma thesis
AuthorFrane Šuman
MentorVedrana Čikeš Čulić (mentor)
Committee memberVedrana Čikeš Čulić (predsjednik povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2017-03-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
Cilj istraživanja bio je ispitati citotoksično djelovanje divlje masline (Olea europaea L. subsp. oleaster), tj. njenog glavnog fenolnog sekoiridoida, oleuropeina. Otopinom oleuropeina koncentracija 0,5 g/L i 1 g/L tretirane su humane stanice karcinoma mokraćnog mjehura (stanična linija T24), dojke (stanična linija MDA- MB-231) i pluća (stanična linija A-549). Citiotoksičnost se određivala nakon 4, 24, 48, i 72 sata MTT metodom. Usporedba apsorbancije nastalog formazana kod tretiranih i netretiranih stanica (kontrola) pokazatelj je postotka preživljenja stanica. In vitro izlaganje karcinomskih stanica oleuropeinu dovelo je do željenog smanjenog preživljenja tih stanica. Citotoksično djelovanje nije jednako na sve ispitivane karcinomske linije, a ovisno je i o koncentraciji otopine oleuropeina te vremenu izlaganja stanica. Smanjeno preživljenje nije uvijek razmjerno s vremenom izlaganja, ali je uočena povezanost s povećanjem koncentracije. Sljedeći korak su in vivo istraživanja na pokusnim životinjama.
Abstract (english)
The aim of this study was to examine citotoxic effect of wild olive (Olea europaea L. subsp. oleaster) and its main phenolic secoiridoid, oleuropein. Urinary bladder cancer cell line T24, breast cancer cell line MDAMB-231 and lung cancer cell line A-549 were treated with 0.5 g/L and 1 g/L oleuropein solution. Cytotoxicity was determined after 4, 24, 48 and 72 hours by MTT test. Percentage of cell survival was determined by comparing the absorbance of formazan in treated and untreated cells (control). In vitro exposure of cancer cells to oleuropein led to aimed cell survival reduction. The reduced survival rate was not equal for every tested cancer line and it depended on the concentration of oleuropein solution and the cell exposure time. Decreased survival was not always proportional to the exposure time, but it was proportional to concentration increase. The next step are in vivo studies on experimental animals.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:697922
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar/ Key words are revised by the librarian
Created on2017-07-10 09:48:40