| Title | Citotoksično djelovanje oleuropeina iz ekstrakta lista divlje masline na različite stanične linije humanih karcinoma mjereno MTT metodom : diplomski rad |
| Author | Frane Šuman |
| Mentor(s) | Vedrana Čikeš Čulić (thesis advisor)
|
| Abstract | Cilj istraživanja bio je ispitati citotoksično djelovanje divlje masline (Olea europaea L. subsp. oleaster), tj. njenog glavnog fenolnog sekoiridoida, oleuropeina. Otopinom oleuropeina koncentracija 0,5 g/L i 1 g/L tretirane su humane stanice karcinoma mokraćnog mjehura (stanična linija T24), dojke (stanična linija MDA- MB-231) i pluća (stanična linija A-549). Citiotoksičnost se određivala nakon 4, 24, 48, i 72 sata MTT metodom. Usporedba apsorbancije nastalog formazana kod tretiranih i netretiranih stanica (kontrola) pokazatelj je postotka preživljenja stanica. In vitro izlaganje karcinomskih stanica oleuropeinu dovelo je do željenog smanjenog preživljenja tih stanica. Citotoksično djelovanje nije jednako na sve ispitivane karcinomske linije, a ovisno je i o koncentraciji otopine oleuropeina te vremenu izlaganja stanica. Smanjeno preživljenje nije uvijek razmjerno s vremenom izlaganja, ali je uočena povezanost s povećanjem koncentracije. Sljedeći korak su in vivo istraživanja na pokusnim životinjama. |
| Keywords | wild olive oleuropein: urinary bladder cancer(T24) breast cancer(MDA-MB-231) lung cancer(A-549) cytotoxicity MTTmethod |
| Parallel title (English) | Cytotoxic activity of oleuropein extracted from wild olive leaf in various human cancer cells lines measured by MTT method : diploma thesis |
| Committee Members | Vedrana Čikeš Čulić (committee chairperson)
|
| Granter | University of Split
Faculty of Chemistry and Technology
Library |
| Granter | University of Split
School of Medicine |
| Place | Split |
| Place | Split |
| State | Croatia |
| Scientific field, discipline, subdiscipline | BIOMEDICINE AND HEALTHCARE
Pharmacy
|
| Study programme type | university |
| Study level | integrated undergraduate and graduate |
| Study programme | Pharmacy |
| Academic title abbreviation | mag. pharm. |
| Genre | master's thesis |
| Language | Croatian |
| Defense date | 2017-03-29 |
| Parallel abstract (English) | The aim of this study was to examine citotoxic effect of wild olive (Olea europaea L. subsp. oleaster) and its main phenolic secoiridoid, oleuropein. Urinary bladder cancer cell line T24, breast cancer cell line MDAMB-231 and lung cancer cell line A-549 were treated with 0.5 g/L and 1 g/L oleuropein solution. Cytotoxicity was determined after 4, 24, 48 and 72 hours by MTT test. Percentage of cell survival was determined by comparing the absorbance of formazan in treated and untreated cells (control). In vitro exposure of cancer cells to oleuropein led to aimed cell survival reduction. The reduced survival rate was not equal for every tested cancer line and it depended on the concentration of oleuropein solution and the cell exposure time. Decreased survival was not always proportional to the exposure time, but it was proportional to concentration increase. The next step are in vivo studies on experimental animals. |
| Parallel keywords (Croatian) | divlja maslina oleuropein karcinom mokraćnog mjehura(T24) rak dojke(MDA-MB-231) karcinom mokraćnog mjehura(T24) karcinom pluća(A-549) citotoksičnost MTT-metoda |
| Resource type | text |
| Access condition | Open access |
| Terms of use |  |
| Note | Ključne riječi doradio knjižničar/ Key words are revised by the librarian |
| URN:NBN | https://urn.nsk.hr/urn:nbn:hr:167:697922 |
