Glucosinolates are secondary metabolites that are synthesized from amino acids. They were found in over 16 plant families, the most important being the Brassicaceae family, which includes the plant Lepidium sativum L. Glucosinolates are chemically and biologically inactive compounds and have in common that they are chemically β-thioglucoside-N-hydroxysulfates with a variable side chain. The Lepidium sativum L. plant is an annual crop that contains many medicinal substances, but is poorly used in the human diet. Cress salad is known to be a good hyperaccumulator of heavy metals. Plants do not need cadmium and generally do not tolerate concentrations higher than 1 ppm, but this plant can accumulate cadmium in tissues and tolerate concentrations up to 100 ppm. This thesis describes the cultivation of the plant Lepidium sativum L., which was watered with cadmium solutions of different concentrations from 1 to 50 ppm. Sprouts development was monitored for 7 days and only at a concentration of 50 ppm did the plant decay. Isolation of glucosinolate was performed by extraction with 70% methanol with desulfation, while the analysis was performed using UHPLC-DAD- MS/MS. The following glucosinolates were identified: glucotropeolin was found in all samples and 4- methoxyglucobrasicin which was not found only in seeds. Glucotropeolin belongs to the group of arylaliphatic, while 4-methoxyglucobrasicin belongs to the group of heterocyclic (indole) glucosinolates. The glucosinolate content, since the samples were watered with cadmium solutions of different concentrations, did not change significantly. The thesis also describes the preparation of plant material for analysis by atomic absorption spectroscopy. Before starting the analysis, it is necessary to make a calibration curve because the cadmium concentrations in the plant material are read through it. Based on the obtained results, it was concluded that the plant Lepidium sativum L. can be used as a hyperaccumulator of cadmium.