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undergraduate thesis
Spektrofotometrijsko određivanje ukupnih fenola u uzorcima algi
završni rad
2020. urn:nbn:hr:167:598369
Žanko, Tea
University of Split
Faculty of Chemistry and Technology
Division of Engineering and Chemistry

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Žanko, T. (2020). Spektrofotometrijsko određivanje ukupnih fenola u uzorcima algi : završni rad (Undergraduate thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:598369

Žanko, Tea. "Spektrofotometrijsko određivanje ukupnih fenola u uzorcima algi : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:167:598369

Žanko, Tea. "Spektrofotometrijsko određivanje ukupnih fenola u uzorcima algi : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2020. https://urn.nsk.hr/urn:nbn:hr:167:598369

Žanko, T. (2020). 'Spektrofotometrijsko određivanje ukupnih fenola u uzorcima algi : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:598369

Žanko T. Spektrofotometrijsko određivanje ukupnih fenola u uzorcima algi : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2020 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:167:598369

T. Žanko, "Spektrofotometrijsko određivanje ukupnih fenola u uzorcima algi : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:167:598369

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TitleSpektrofotometrijsko određivanje ukupnih fenola u uzorcima algi : završni rad
Title (english)Spectrophotometric determination of total phenolics in seaweeds : bachelor thesis
AuthorTea Žanko
MentorLadislav Vrsalović (mentor)
Committee memberDanijela Skroza (predsjednik povjerenstva)
Committee memberMario Nikola Mužek (član povjerenstva)
Committee memberLadislav Vrsalović (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Engineering and Chemistry)
Split
Defense date and country2020-10-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Alge su važan izvor različitih bioaktivnih spojeva, stoga su od iznimne važnosti otkrića i razvoj novih analitičkih metoda i tehnika za proučavanje njihovih metabolita. Osobitu pozornost znanstvenika privukli su fenoli smeđih algi koji su karakteristični samo za te organizme, a nazivaju se florotanini. Cilj ovog završnog rada je bio postaviti metodu određivanja ukupnih fenola na dva uređaja; klasičnom UV-VIS spektrofotometru i mikrotitarskom čitaču ploča. Kod postavljanja metode korištene su dvije otopine standarda, galna kiselina i floroglucinol. Galna kiselina je općenito najkorišteniji standard za izradu baždarnih pravaca i izražavanje rezultata određivanja fenola u različitim uzorcima (biljke, plodovi, sokovi, prerađevine, itd.), dok je floroglucinol osnovna građevna jedinica florotanina. Obzirom da se rezultati određivanja sadržaja fenola u algama u znanstvenim studijama izražavaju preko oba spoja, postavljanje metode određivanja ukupnih fenola korištenjem oba standarda može olakšati njihovu međusobnu usporedbu.
Abstract (english)
Seaweeds are an important source of various bioactive compounds, so discovery and development of new analytical methods and techniques for the study of their metabolites are extremely important. Special attention has been drawn to the brown algae phenolics, which are unique to these organisms and are called phlorotanins. The aim of this bachelor thesis was to set up a method for the determination of total phenolics using two devices; classic UV-VIS spectrophotometer and microtiter plate reader. Two standard solutions, gallic acid and phloroglucinol, were used in setting up the method. Gallic acid is generally the most widely used standard for creation of calibration curves and determination of phenols in various samples (plants, fruits, juices, processed products, etc.), while phloroglucinol is the basic building unit of phlorotanins. Since in the scientific literature the phenolic content of seaweed samples is expressed using both standards, setting up a method for determining total phenols using both standards can provide easier comparison of the results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:598369
Study programmeTitle: Chemical Technology; specializations in: Chemical Engineering, Environmental Protection
Course: Chemical Engineering
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva (sveučilišni/a prvostupnik/ prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2021-01-18 08:19:06