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undergraduate thesis
Antiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.)
završni rad
2019. urn:nbn:hr:167:678888
Mijoč, Domagoj
University of Split
Faculty of Chemistry and Technology
Library

Cite this document

Mijoč, D. (2019). Antiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.) : završni rad (Undergraduate thesis). Split: University of Split, Faculty of Chemistry and Technology, Library. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:678888

Mijoč, Domagoj. "Antiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.) : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Library, 2019. https://urn.nsk.hr/urn:nbn:hr:167:678888

Mijoč, Domagoj. "Antiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.) : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Library, 2019. https://urn.nsk.hr/urn:nbn:hr:167:678888

Mijoč, D. (2019). 'Antiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.) : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Library, accessed 23 April 2024, https://urn.nsk.hr/urn:nbn:hr:167:678888

Mijoč D. Antiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.) : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology, Library; 2019 [cited 2024 April 23] Available at: https://urn.nsk.hr/urn:nbn:hr:167:678888

D. Mijoč, "Antiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.) : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Library, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:167:678888

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Metadata
TitleAntiproliferativna aktivnost mikrovalnih ekstrakata sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L.) : završni rad
Title (english)Antiproliferative activity of wild (Sinapis arvensis L.) and white (Sinapis alba L.) microwave mustard seeds extracts : bachelor thesis
AuthorDomagoj Mijoč
MentorTea Bilušić (mentor)
MBZ: 238765
Committee memberDanijela Skroza (predsjednik povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
Library
Split
Defense date and country2019-10-02, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Glukozinolati (β-tioglukozid-N-hidroksisulfati) su skupina sekundarnih metabolita koji su zastupljeni u 16 botaničkih porodica reda Brassicales. Biološki su neaktivni, a međusobno se razlikuju po strukturi bočnog lanca. Djelovanjem enzima mirozinaze razlažu se na biološki aktivne metabolite poput izotiocijanata i nitrila. Izotiocijanati su hlapljivi sumporovi spojevi koji nastaju hidrolizom glukozinolata pri neutralnoj pH vrijednosti, a po kemijskom sastavu su esteri izotiocijanatne kiseline. Pokazuju antioksidativnu, antiupalnu, antimikrobnu, neurozaštitnu, kardiozaštitnu i antikancerogenu aktivnost. Glukozinolati i njihovi razgradni produkti odgovorni su za karakterističan miris i aromu gorušice i proizvoda od gorušice. Pri izradi rada korišteni su mikrovalni ekstrakti i destilati sjemenki divlje (Sinapis arvensis L.) i bijele gorušice (Sinapis alba L), a kemijski sastav uzoraka određen je GC-MS analizom. Analizom nisu detektirani razgradni produkti glukozinolata ni u jednom uzorku što ne znači da i nisu prisutni. Citotoksičan učinak uzoraka određen je primjenom MTT metode na staničnim linijama MBA-MB-231 (adenokarcinom dojke) i TCCSUP (karcinom prijelaznih stanica mokraćnog mjehura). Svi uzorci pokazuju citotoksičan učinak na MBA-MB-231 staničnu liniju, ali on nije nužno proporcionalan koncentraciji i trajanju inkubacije. Na staničnu liniju TCCSUP znatniji citotoksičan učinak imao je samo mikrovalni ekstrakt divlje gorušice, a u određenim slučajevima došlo je i do porasta broja stanica karcinoma.
Abstract (english)
Glucosinolates (β-thioglucoside-N-hydroxysulfates) are secondary plant metabolites found in 16 botanical families of the Brassicales order. They are biologically inactive and differ by side chain structure. Myrosinase enzymes hydrolyze glucosinolates to their biologically active metabolites, such as isothiocyanates and nitriles. Isothiocyanates are volatile sulphur containing compounds which are formed by hydrolysis in pH neutral environment and are isothiocyanic acid esters by their chemical composition. They show antioxidant, anti-inflammatory, neuroprotective, cardioprotective and anticancer activity. Glucosinolates and their breakdown products are responsible for characteristic odor and aroma of mustard and mustard products. Microwave extracts and distillates of wild (Sinapis arvensis L.) and white (Sinapis alba L.) mustard seeds were used in the preparation of the paper and their chemical composition was determined by GC-MS analysis. Glucosinolate breakdown products were not detected in any sample, but that doesn’t mean that they aren’t present. Cytotoxic activity was determined by MTT assay on MBA-MB-231 (breast adenocarcinoma) and TCCSUP (urinary bladder transitional call carcinoma) cancer cell lines. All samples express cytotoxic activity on MBA-MB-231 cell line, but not necessarily proportional with sample concentration and incubation time. Only wild mustard microwave extract has had more significant effect on TCCSUP cell line and in some cases increase in number of cancer cells can be seen.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:678888
ProjectNumber: 1316
Title: Biljke kao izvor bioaktivnih sumporovih spojeva te njihova sposobnostt hiperakumulacije metala
Acronym: BioSMe
Leader: Ivica Blažević
Jurisdiction: Croatia
Funder: HRZZ
Study programmeTitle: Undergrasuate Study of Food Technology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik (baccalaureus) inženjer prehrambene tehnologije (sveučilišni prvostupnik (baccalaureus) inženjer prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-02-04 12:15:33