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undergraduate thesis
Prirodna fluorescencija kod bakterija roda Legionella
završni rad
2019. urn:nbn:hr:167:046143
Trtanj, Mia
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

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Trtanj, M. (2019). Prirodna fluorescencija kod bakterija roda Legionella : završni rad (Undergraduate thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:046143

Trtanj, Mia. "Prirodna fluorescencija kod bakterija roda Legionella : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:167:046143

Trtanj, Mia. "Prirodna fluorescencija kod bakterija roda Legionella : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2019. https://urn.nsk.hr/urn:nbn:hr:167:046143

Trtanj, M. (2019). 'Prirodna fluorescencija kod bakterija roda Legionella : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, accessed 16 November 2024, https://urn.nsk.hr/urn:nbn:hr:167:046143

Trtanj M. Prirodna fluorescencija kod bakterija roda Legionella : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2019 [cited 2024 November 16] Available at: https://urn.nsk.hr/urn:nbn:hr:167:046143

M. Trtanj, "Prirodna fluorescencija kod bakterija roda Legionella : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:167:046143

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Metadata
TitlePrirodna fluorescencija kod bakterija roda Legionella : završni rad
Title (english)Natural fluorescence of bacteria of the genus Legionella : bachelor thesis
AuthorMia Trtanj
MentorMila Radan (mentor)
MBZ: 247111
Committee memberTea Bilušić (predsjednik povjerenstva)
Committee memberOlivera Politeo (član povjerenstva)
Committee memberMila Radan (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2019-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Završni rad predstavlja ispitivanje uzoraka vodovodne vode u koje su dodane ciljane koncentracije bakterijskog soja Legionella bozemanii (ATCC 33217 Fluoribacter bozemanae). Uzorkovanje je provedeno prema standardu BS 7592:2008 (odjeljak 2; rutinsko uzimanje uzoraka), a sama analiza vode prema normi HRN EN ISO/IEC 11731:2017. Svaki uzorak procesiran je na dvije vrste selektivnih hranjivih podloga (BCYE i GVPC) i obrađen s 14 različitih procedura. Nakon 4 dana provedene inkubacije na 36 °C (uz 3% CO 2 ) obavljeno je prvo očitavanje/ pregled nasađenih uzoraka. Legionella bozemanii, kao i određeni ostali sojevi, pokazuju auto- fluorescenciju kada se promatraju ultraljubičastim svjetlom. Upravo ta karakteristika ispitivane bakterije omogućila je njezinu detekciju pomoću ručne fluorescentne lampe na hranjivim podlogama. Nakon detekcije fluorescentnih sojeva dodatna potvrda napravljena je nasađivanjem istih na BCYE hranjive podloge (uz ili bez dodataka aminokiseline cisteina.) Konačni rezultati nakon 10 dana inkubacije jasno su pokazali da je brojnost legionela na hranjivim podlogama sukladna dodanim koncentracijama L. bozemanii
Abstract (english)
The final study presents an examination of tap/potable water samples to which targeted concentrations of the bacterial strain Legionella bozemanii (ATCC 33217 Fluoribacter bozemanae) were added. Sampling was performed according to BS 7592; 2008 (Section 2; Routine sampling) and the water analysis was performed acording to norm HRN EN ISO/ IEC 11731: 2017. Each sample was processed on two types of selective nutrient media (BCYE and GVPC) andprocessed with 14 different procedures. After 4 days of incubation at 36 °𝐶 (with 3% CO 2 ), the first reading / examination of the specimens was performed. Legionella bozemanii, like certain other strains, exhibit auto-fluorescence when viewed with the ultraviolet light. It is the characteristic of the tested bacterium that enabled us to detect it with the help of a hand-held fluorescent lamp on prepared nutirent media. After detection of fluorescent strains, additional confirmation was made by inoculation on BCYE nutrient media (with or witohout the addition of the cysteine amino acid). The final results after 10 days of incubation period clearly showed that the abundance (CFU) of legionella on nutrient media was consistent with the added concentrations of L. bozemanii.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:046143
Study programmeTitle: Chemistry
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-01-31 11:23:56