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master's thesis
Određivanje masnih kiselina GC-FID tehnikom
diplomski rad
2024. urn:nbn:hr:167:082268
Boras, Dorja
University of Split
Faculty of Chemistry and Technology
Division of Chemistry
Department of Analytical Chemistry

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Boras, D. (2024). Određivanje masnih kiselina GC-FID tehnikom : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:082268

Boras, Dorja. "Određivanje masnih kiselina GC-FID tehnikom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:167:082268

Boras, Dorja. "Određivanje masnih kiselina GC-FID tehnikom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:167:082268

Boras, D. (2024). 'Određivanje masnih kiselina GC-FID tehnikom : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 02 April 2025, https://urn.nsk.hr/urn:nbn:hr:167:082268

Boras D. Određivanje masnih kiselina GC-FID tehnikom : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2024 [cited 2025 April 02] Available at: https://urn.nsk.hr/urn:nbn:hr:167:082268

D. Boras, "Određivanje masnih kiselina GC-FID tehnikom : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:167:082268

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Metadata
TitleOdređivanje masnih kiselina GC-FID tehnikom : diplomski rad
Title (english)Determination of fatty acids by GC-FID technique : diploma thesis
AuthorDorja Boras
MentorFranko Burčul (mentor)
Committee memberIvana Škugor Rončević (predsjednik povjerenstva)
Committee memberAzra Đulović (član povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
(Department of Analytical Chemistry)
Split
Defense date and country2024-10-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Masne kiseline sastoje se od ravnih lanaca s parnim brojem ugljikovih atoma s karboksilnom skupinom. Grade lipide, ključna su komponenta u membranama stanica te sudjeluju u prijenosu tvari i signala u stanicama živih bića. Masne kiseline mogu se podijeliti s obzirom na zasićenost. Zasićene masne kiseline sadržavaju isključivo jednostruke veze između ugljikovih atoma, dok nezasićene masne kiseline imaju jednu ili više dvostrukih veza. Višestruko nezasićene masne kiseline koje ljudski... More organizam ne može sam sintetizirati nazivaju se esencijalnim masnim kiselinama. To su linolna, linolenska i arahidonska masna kiselina te ih je potrebno unositi hranom. Od esencijalnih masnih kiselina se u organizmu stvaraju nizovi omega masnih kiselina koje su od iznimne važnosti za normalno funkcioniranje organizma. Masne kiseline određuju se u obliku njihovih metilnih estera te je u tu svrhu korištena smjesa 37 metilnih estera masnih kiselina kao standard. Metoda za odjeljivanje te kvalitativno i kvantitativno određivanje razvijena je korištenjem plinske kromatografije uz plamenoionizacijski detektor (GC-FID). Metoda preporučena od strane proizvođača kolone modificirana je i optimizirana promjenom temperaturnog programa kako bi se uspješno odijelilo 37 estera masnih kiselina. Ispitivani parametri vrednovanja metode (raspon, linearnost, granica dokazivanja i određivanja, točnost, ponovljivost, srednja preciznost te prikladnost sustava) pokazali su se zadovoljavajućima. Metoda je primijenjena i na uzorke jestivih ulja te standarde masnih kiselina. Rezultati realnih uzoraka ukazali su na važan korak pripreme uzorka te je uočeno da je nužno poboljšati korak esterifikacije masnih kiselina u uljima. Less
Abstract (english)
Fatty acids consist of straight chains with an even number of carbon atoms with a carboxyl group. They build lipids, are a key component in cell membranes and participate in the transmission of substances and signals in the cells of living beings. Fatty acids can be divided according to saturation. Saturated fatty acids contain only single bonds between carbon atoms, while unsaturated fatty acids have one or more double bonds. Polyunsaturated fatty acids that the human body cannot... More synthesize on its own are called essential fatty acids. These are linoleic, linolenic and arachidonic fatty acids and must be consumed with food. From essential fatty acids, series of omega fatty acids are created in the body, which are extremely important for the normal functioning of the body. Fatty acids are determined in the form of their methyl esters, and for this purpose a mixture of 37 methyl esters of fatty acids was used as a standard. The method for separation and qualitative and quantitative determination was developed using gas chromatography with a flame ionization detector (GC-FID). The method recommended by the column manufacturer was modified and optimized by changing the temperature program to successfully separate 37 fatty acid esters. The examined parameters of the evaluation of the method (range, linearity, limit of proof and determination, accuracy, repeatability, mean precision and suitability of the system) proved to be satisfactory. The method was also applied to edible oil samples and fatty acid standards. The results of real samples indicated an important step of sample preparation and it was observed that it is necessary to improve the step of esterification of fatty acids in oils. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:082268
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2025-01-23 09:18:30
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