prikaz prve stranice dokumenta Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju
Embargo period 2026-06-09
master's thesis
Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju
diplomski rad
2023. urn:nbn:hr:167:833558
Usanović, Katarina
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

Cite this document

Usanović, K. (2023). Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:833558

Usanović, Katarina. "Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:833558

Usanović, Katarina. "Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2023. https://urn.nsk.hr/urn:nbn:hr:167:833558

Usanović, K. (2023). 'Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 01 April 2025, https://urn.nsk.hr/urn:nbn:hr:167:833558

Usanović K. Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2023 [cited 2025 April 01] Available at: https://urn.nsk.hr/urn:nbn:hr:167:833558

K. Usanović, "Određivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:167:833558

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Metadata
TitleOdređivanje glukozinolata u Biscutella laevigata i Eruca vesicaria te primjena ubrzane ekstrakcije otapalima za njihovu izolaciju : diplomski rad
Title (english)Determination of glucosinolates in Biscutella laevigata and Eruca vesicaria and their isolation using accelerated solvent extraction : diploma thesis
AuthorKatarina Usanović
MentorIvica Blažević (mentor)
Committee memberMario Nikola Mužek (predsjednik povjerenstva)
Committee memberIvica Blažević (član povjerenstva)
Committee memberAzra Đulović (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2023-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U ovom radu određen je glukozinolatni profil sjemena rokule (Eruca vesicaria) i odabranih biljnih dijelova biokovske i poljske glatke dvoštitke (Biscutella laevigata) izravnom UHPLC-DAD-MS/MS analizom preko odgovarajućih desulfoglukozinolata. Ekstrakcija glukozinolata provedena je sukladno modificiranoj ISO 9167-1 metodi. Dodatno, glukozinolati sjemena rokule izolirani su metodom ubrzane ekstrakcije otapalom pri povišenom tlaku (ASE) s ciljem kvalitativne i kvantitativne... More usporedbe dobivenih glukozinolata s ISO metodom. Ispitana je ekstrakcijska učinkovitost ASE na temperaturama od 50 ºC, 70 ºC i 90 ºC i s ulaznim količinama biljnog materijala od 1 g i 100 mg. Analizom sjemena rokule, neovisno o primijenjenoj metodi izolacije, dokazana je prisutnost glukoerucina u najvišim koncentracijama (6,13-90,24 μmol/g suhog biljnog materijala). U dosta nižem sadržaju pronađeni su glukorafanin (0,14-1,46 μmol/g) i glukosinalbin (0,13-0,65 μmol/g), a ostali glukozinolati su prisutni u tragovima. Pojedinačni i ukupni (92,35 μmol/g) sadržaji glukozinolata pokazali su se značajno višim primjenom ISO 9167-1 metode. Najveći sadržaj glukozinolata za ASE dobiven je pri temperaturi 70 ºC, tj. 32,57 μmol/g za 1 g i 7,80 μmol/g za 100 mg. Glukohirsutin i glukobrasicin su pronađeni u svim biljnim dijelovima biokovske glatke dvoštitke, a najveći sadržaj bio je u komuški (43,57 μmol/g za glukohirsutin i 14,08 μmol/g za glukobrasicin). Općenito, komuška se pokazala najbogatijom glukozinolatima (57,85 μmol/g), a najsiromašnija je bila stabljika (6,49 μmol/g). Analizom poljske glatke dvoštitke, glukozinolati su ustanovljeni samo u sjemenu unutar kojeg je najsadržajniji bio glukohirsutin (30,51 μmol/g), dok uzorak dobiven in vitro nije sadržavao glukozinolate. Less
Abstract (english)
In this paper, the glucosinolate profile of rocket seeds (Eruca vesicaria) and selected plant parts of Biokovo and Polish buckler mustard (Biscutella laevigata) was determined using UHPLC-DAD-MS/MS analysis by their desulfo-counterparts. Glucosinolate extraction was performed according to the modified ISO 9167-1 method. Additionally, the glucosinolates of rocket seeds were isolated by accelerated solvent extraction at elevated pressure (ASE) with the aim of comparing the ... More qualitative and quantitative glucosinolate profile with the ISO method. The extraction efficiency of ASE was tested at temperatures of 50 ºC, 70 ºC and 90 ºC with the amounts of plant material of 1 g and 100 mg. The analysis of rocket seeds, regardless of the applied isolation method, revealed the presence of glucoerucin having the highest content (6.13-90.24 μmol/g of dried plant material). Glucoraphanin (0.14- 1.46 μmol/g) and glucosinalbin (0.13-0.65 μmol/g) were found in much lower concentrations, and other glucosinolates were present in traces. Individual and total (92.35 μmol/g) glucosinolate contents were shown to be significantly higher using the ISO 9167-1 method. The highest yield for ASE was obtained at 70 ºC with total glucosinolate content of 32.57 μmol/g for 1 g and 7.80 μmol/g for 100 mg. Glucohirsutin and glucobrassicin were found in all plant parts of buckler mustard from Biokovo and the highest contents were found in siliquae (43.57 μmol/g for glucohirsutin and 14.08 μmol/g for glucobrassicin). In general, siliquae proved to be the richest in glucosinolates (57.85 μmol/g), while the stem had the lowest (6.49 μmol/g). By analyzing the Polish buckler mustard, glucosinolates were found only in the seeds, the most abundant of which was glucohirsutin (30.51 μmol/g), while the glucosinolates were absent in the plant sample obtained in vitro. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:833558
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-06-09
Terms of useLicence In copyright icon
Created on2024-05-10 07:20:38
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