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master's thesis
Utjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom
diplomski rad
2016. urn:nbn:hr:167:134876
Šarić, Mia
University of Split
Faculty of Chemistry and Technology
Division of Chemistry
University of Split
School of Medicine

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Šarić, M. (2016). Utjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:134876

Šarić, Mia. "Utjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:167:134876

Šarić, Mia. "Utjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:167:134876

Šarić, M. (2016). 'Utjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 28 October 2024, https://urn.nsk.hr/urn:nbn:hr:167:134876

Šarić M. Utjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2016 [cited 2024 October 28] Available at: https://urn.nsk.hr/urn:nbn:hr:167:134876

M. Šarić, "Utjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:167:134876

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Metadata
TitleUtjecaj taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenom kroničnom hiperglikemijom : diplomski rad
Title (english)The influence of the taninic acid on the expression of the connexions 45 in a rat kidney damaged by the chronic hyperglycemia : master's thesis
AuthorMia Šarić
MentorNatalija Filipović (mentor)
Committee memberKatarina Vukojević (predsjednik povjerenstva)
Committee memberSandra Kostić (član povjerenstva)
Committee memberNatalija Filipović (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
GranterUniversity of Split
School of Medicine
Split
Defense date and country2016-10-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Abstract
Cilj ovog diplomskog rada je dokazati potencijalni negativan učinak taninske kiseline na izražaj koneksina 45 u bubregu štakora oštećenog kroničnom hiperglikemijom, kako bi se što bolje mogli kritički osloniti na povoljna djelovanja i uporabu taninske kiseline u liječenju mnogobrojnih bolesti. Istraživanje koje je trajalo dvadeset tjedana provedeno je na dvanaest muških štakora soja Wistar. Štakori su podijeljeni u tri skupine po četiri štakora. Skupine su formirane na osnovu napitaka koje su štakori konzumirali. Prema dobivenim rezultatima 20-tog tjedna eksperimenta uočeno je da je skupina štakora koji su konzumirali saharozu u pitkoj vodi (30%) imala značajan porast glukoze u krvi u odnosu na kontrolnu grupu, a porast glukoze u krvi je bio manji dodatkom taninske kiseline (0,1%). Dodatak taninske kiseline (0,1% u vodi za piće; Sah/TK skupina) rezultirao je smanjenjem tjelesne mase štakora. Patohistološki nalaz ukazuje na oštećenja bubrega uzrokovana hiperglikemijom, dok su oštećenja jače izražena u Sah/TK skupini. Analizom mikrofotografija utvrdili smo da hiperglikemija uzrokuje smanjeni izražaj koneksina 45 u proksimalnim zavijenim kanalićima bubrega, a dodatkom taninske kiseline to smanjenje postaje statistički značajno (p<0,05). Temeljem dobivenih rezultata zaključili smo da taninska kiselina u kombinaciji s kroničnom hiperglikemijom u primjenjenoj dozi od 0.1% u vodi za piće oštećuje bubrege, te rezultira značajnim padom izražaja koneksina 45 u proksimalnim zavijenim kanalićima štakora.
Abstract (english)
The aim of this thesis was to demonstrate the potential negative effect of the tannic acid on the expression of connexin 45 in rat kidney damaged by the chronic hyperglycemia, due to a need for critical assessment of the positive effects of the use of tannic acid in the treatment of many diseases. Twelve male Wistar rats were used in the trial over a period of twenty weeks. The rats were divided into three groups of four rats. The groups were formed based on beverages consumed by the rats. At the end of this 20-week experiment it was observed that the group of rats that consumed sucrose in drinking water (30%) had a significant increase in blood glucose level compared to the control group which consumed drinking water. In the third group, where tannic acid along with the sucrose (0,1% of tannic acid; Sah/TK group) was added in a drinking water, blood glucose levels were less increased than in the group that consumed sucrose in water. Also consumption of tannic acid (0,1%) had a positive effect on the weight reduction. Pathohistologically, we observed damage of the kidneys caused by the hyperglycemia, while damage was more expressed in the Sah/TK group. Analysis of the microphotographs showed that hyperglycemia caused reduced expression of connexin 45 in the proximal tubules of the kidney, and the addition of the tannic acid made the reduction statistically significant (p <0.05). Based on the results, we concluded that the tannic acid in combination with the chronic hyperglycemia, in the dosage of 0,1% in drinking water, damages the kidneys. We also concluded that tannic acid in combination with the chronic hyperglycemia significantly decreases the expression of the connexin 45 in the proximal tubules of the rat kidney.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:134876
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-11-22 13:11:37