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undergraduate thesis
Antioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity)
završni rad
2016. urn:nbn:hr:167:244976
Mišković, Ana
University of Split
Faculty of Chemistry and Technology
Division of Engineering and Chemistry

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Mišković, A. (2016). Antioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity) : završni rad (Undergraduate thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:244976

Mišković, Ana. "Antioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity) : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:244976

Mišković, Ana. "Antioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity) : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:244976

Mišković, A. (2016). 'Antioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity) : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, accessed 13 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:244976

Mišković A. Antioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity) : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2016 [cited 2024 December 13] Available at: https://urn.nsk.hr/urn:nbn:hr:167:244976

A. Mišković, "Antioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity) : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:167:244976

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Metadata
TitleAntioksidacijske metode: metoda ORAC (engl. Oxygen Radical Absorbance Capacity) : završni rad
Title (english)Antioxidant methods: ORAC assay (Oxygen Radical Absorbance Capacity) : bachelor thesis
AuthorAna Mišković
MentorIvana Generalić Mekinić (mentor)
Committee memberMario Nikola Mužek (predsjednik povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
Committee memberIvana Generalić Mekinić (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Engineering and Chemistry)
Split
Defense date and country2016-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		TECHNICAL SCIENCES
Chemical Engineering
Abstract
Zbog brojnosti i kemijske raznolikosti molekula antioksidansa, različitih mehanizama njihova djelovanja, kao i ciljanih molekula na koje se želi ispitati njihov utjecaj, do danas je razvijen veliki broj antioksidacijskih metoda. Kako se niti jedna od postojećih metoda nije pokazala prikladnom da u potpunosti reflektira antioksidacijski kapacitet i svojstva uzorka, uobičajeno je da se u istraživanjima koristi nekoliko metoda koje se temelje na različitim reakcijskim mehanizmima. Ipak, veliki broj metoda i njihove modifikacije često dovode do nepravilne interpretacije rezultata i njihove otežane rezultata pa se javlja potreba za standardiziranom metodom koja bi omogućila jednoznačno mjerenje antioksidacijskog kapaciteta uzoraka. ORAC (engl. Oxygen Radical Absorbance Capacity) metoda je općeprihvaćena standardizirana antioksidacijska metoda. Prema mehanizmu reakcije koji se odvija kod ORAC metode ona spada u antioksidacijske metode koje se temelje na prijenosu atoma vodika. Rezultati, odnosno dobivene ORAC vrijednosti, uobičajeno se izražavaju preko Troloks ekvivalenta, u jedinicama µ M TE/g ili µ M TE/L uzorka, što znatno olakšava usporedbu rezultata različitih istraživanja.
Abstract (english)
Due to the abundance and chemical diversity of antioxidant molecules, their different mechanisms of action, as well as the final target molecules, a large number of antioxidant methods have been developed. As one antioxidant method can not describe the antioxidant properties and total antioxidant capacity of the sample, multiple-method approach, using methods based on different reaction mechanisms, is usually performed. However, a great number of methods and their modifications often lead to an incorrect interpretation of the results and caused problems in their comparison. Therefore, there is a need for a standardized method that would allow an unambiguous determination of antioxidant capacity of the samples. ORAC (Oxygen Radical Absorbance Capacity) assay is widely accepted and standardized antioxidant method. The mechanism of the reaction that takes place in the ORAC method is hydrogen atom transfer. The obtained results, or so called ORAC values, are usually expressed as Trolox equivalents, in µ M TE/g or µ M TE/L, what greatly allows the comparison of results from different studies.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:244976
ProjectNumber: IP-2014-09-6897
Title: Istraživanje bioaktivnih spojeva iz dalmatinskogbilja: njihov antioksidacijski karakter i utjecaj na enzimsku inhibiciju i zdravlje
Acronym: BioActCom
Leader: Mladen Miloš
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Chemical Technology; specializations in: Chemical Technology and Materials, Food Technology
Course: Chemical Technology and Materials
Study programme type: professional
Study level: undergraduate
Academic / professional title: stručni/a pristupnik/pristupnica kemijskog inženjerstva (stručni/a pristupnik/pristupnica kemijskog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-04-25 09:34:29