prikaz prve stranice dokumenta Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum)
Embargo period 2027-01-23
master's thesis
Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum)
diplomski rad
2024. urn:nbn:hr:167:634805
Petrović, Jakov
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

Cite this document

Petrović, J. (2024). Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum) : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:634805

Petrović, Jakov. "Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum) : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:167:634805

Petrović, Jakov. "Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum) : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2024. https://urn.nsk.hr/urn:nbn:hr:167:634805

Petrović, J. (2024). 'Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum) : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 23 January 2025, https://urn.nsk.hr/urn:nbn:hr:167:634805

Petrović J. Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum) : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2024 [cited 2025 January 23] Available at: https://urn.nsk.hr/urn:nbn:hr:167:634805

J. Petrović, "Profil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum) : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:167:634805

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Metadata
TitleProfil glukozinolata i spektroskopska analiza izoliranih desulfoglukozinolata iz hrvatskog šeboja (Erysimum croaticum) : diplomski rad
Title (english)Glucosinolate profile and spectroscopic analysis of isolated desulfoglucosinolates from croatian wallflower (Erysimum croaticum) : diploma thesis
AuthorJakov Petrović
MentorIvica Blažević (mentor)
Committee memberFranko Burčul (predsjednik povjerenstva)
Committee memberAzra Đulović (član povjerenstva)
Committee memberIvica Blažević (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2024-10-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Glukozinolati su specijalizirani biljni metaboliti usko povezani s porodicom Brassicaceae. Struktura se sastoji od β- D -glukopiranoze i O-sulfatirane (Z)-tiohidroksimatne skupine te promjenjivog bočnog lanca. Djelovanjem enzima mirozinaze nastaju razgradni produkti, poznati po brojnim biološkim aktivnostima, od kojih je najzanimljivije njihovo antikancerogeno djelovanje. U ovom radu određen je glukozinolatni profil hrvatskog šeboja (Erysimum croaticum), endemske biljke s Biokova, korištenjem UHPLC-DAD-MS/MS tehnike. Glukozinolati su izolirani u desulfatiranom obliku pomoću ion- izmjenjivačkih kolona prema metodi ISO 9167-1. Analizom MS2 i UV spektara dobivene su kvalitativne i kvantitativne informacije o glukozinolatnom profilu biljke. Ovaj profil je jedinstven za sve biljne organe i sastoji se od dva glukozinolata: glukorafenina i (3E)-4-(metilsulfanil)but-3-enil-glukozinolat. Cvat sadrži najveću koncentraciju glukozinolata (679,74 µmol/g suhog biljnog materijala), dok stabljika ima najmanju (123,85 µmol/g suhog biljnog materijala). Glukorafenin je dominantan u većini biljnih organa, osim u korijenu. Dva analizirana desulfoglukozinolata imaju dovoljno različito vrijeme zadržavanja što omogućava odvajanje na dvije frakcije koje sadržavaju pojedinačne desulfoglukozinolate. Strukture izoliranih desulfoglukozinolata su potvrđene NMR spektroskopijom.
Abstract (english)
Glucosinolates are specialized plant metabolites closely related to the Brassicaceae family. The structure consists of β- D -glucopyranose and O-sulfated (Z)-thiohydroximate group and a variable side chain. Degradation products are produced by the action of the enzyme myrosinase, and are known for numerous biological activities, with their anticarcinogenic effects being the most interesting. In this study, the glucosinolate profile of Croatian wallflower (Erysimum croaticum), an endemic plant from Biokovo, was determined using UHPLC-DAD-MS/MS technique. Glucosinolates were isolated in desulfated form using ion-exchange columns according to the ISO 9167-1 method. Qualitative and quantitative information about the plant's glucosinolate profile was obtained by analyzing MS2 and UV spectra. This profile is unique for all plant organs and consists of two glucosinolates: glucoraphenin and (3E)-4- (methylsulfinyl)but-3-enyl-glucosinolate. The flower contains the highest concentration of glucosinolates (679.74 µmol/g of dry weight), while the stem has the lowest (123.85 µmol/g of dry weight). Glucoraphenin is dominant in most plant organs, except in the root. The two analyzed desulfoglucosinolates have sufficiently different retention times allowing separation into two fractions containing individual desulfoglucosinolates. The structures of isolated desulfoglucosinolates were confirmed by NMR spectroscopy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:634805
Study programmeTitle: Chemistry; specializations in: Environmental Protection, Organic Chemistry and Biochemistry
Course: Environmental Protection
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2027-01-23
Terms of useLicence In copyright icon
Created on2025-01-23 11:24:34