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master's thesis
Utjecaj staništa na kemijski sastav i biološka svojstva motra
diplomski rad
2021. urn:nbn:hr:167:364348
Mimica, Nina
University of Split
Faculty of Chemistry and Technology
Division of Engineering and Chemistry

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Mimica, N. (2021). Utjecaj staništa na kemijski sastav i biološka svojstva motra : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:364348

Mimica, Nina. "Utjecaj staništa na kemijski sastav i biološka svojstva motra : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:167:364348

Mimica, Nina. "Utjecaj staništa na kemijski sastav i biološka svojstva motra : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology, 2021. https://urn.nsk.hr/urn:nbn:hr:167:364348

Mimica, N. (2021). 'Utjecaj staništa na kemijski sastav i biološka svojstva motra : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology, accessed 16 December 2024, https://urn.nsk.hr/urn:nbn:hr:167:364348

Mimica N. Utjecaj staništa na kemijski sastav i biološka svojstva motra : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2021 [cited 2024 December 16] Available at: https://urn.nsk.hr/urn:nbn:hr:167:364348

N. Mimica, "Utjecaj staništa na kemijski sastav i biološka svojstva motra : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:167:364348

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Metadata
TitleUtjecaj staništa na kemijski sastav i biološka svojstva motra : diplomski rad
Title (english)Influence of harvest location on chemical composition and biological activity of sea fennel : diploma thesis
AuthorNina Mimica
MentorIvana Generalić Mekinić (mentor)
Committee memberMario Nikola Mužek (predsjednik povjerenstva)
Committee memberZvonimir Marijanović (član povjerenstva)
Committee memberIvana Generalić Mekinić (član povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Engineering and Chemistry)
Split
Defense date and country2021-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Motar (Crithmum maritimum L.) je vjerojatno jedan od najpoznatijih halofita na području Mediterana. Ova biljka se od davnina koristi u prehrambene i ljekovite svrhe obzirom da je odlikuje prisutnost visokih koncentracija vitamina C, polifenola, karotenoida te ostalih bioaktivnih spojeva. Cilj ovog rada bio je istražiti razlike u kemijskom sastavu i biološkim svojstvima motra prikupljenog sa tri različite lokacije; na riječnom staništu, staništu na samom ušću te na morskom staništu. Korištenjem uređaja za mikrovalnu ekstrakciju pripravljeni su biljni ekstrakti (94°C, 500 W, 30 min) kojima je određen udio ukupnih fenola, flavonoida te ukupnih tanina, dok je sadržaj klorogenske kiseline, koja je dominantan fenolni spoj u motru, određen primjenom visokodjelotvorne tekućinske kromatografije. Uzorcima je testirana i antioksidacijska aktivnost metodom određivanja redukcijske aktivnosti uzoraka (engl. Ferric Reducing Antioxidant Power, FRAP) te korištenjem dviju metoda određivanja sposobnosti hvatanja molekula slobodnog radikala pri čemu je korišten dušikov monoksid (NO • ) i 2,2-difenil-1-pikrilhidrazil radikal (DPPH). Na temelju dobivenih rezulata može se zaključiti da lokacija uzorkovanja biljke motar ima veliki utjecaj na kemijski sastav i antioksidacijska svojstva ekstrakata. Također, rezultati ukazuju na važnost individualnih fenolnih spojeva (klorogenske kiseline) na biološka svojstva ekstrakata.
Abstract (english)
Sea fennel (Crithmum maritimum L.) is probably one of the most known halophytes in the Mediterranean region. This plant has been used from the earliest times for nutritional and medicinal purposes because it is characterized by a high concentration of vitamin C, polyphenols, carotenoids, and other bioactive compounds. The aim of this study was to explore the differences in chemical composition and biological properties of sea fennel collected from three different locations: river habitat, estuarine habitat, and marine habitat. Extracts of sea fennel were prepared by microwave-assisted extraction (94°C, 500 W, 30 min). Obtained extracts were tested for total phenolics, flavonoids, and tannins, while the content of chlorogenic acid, which is the dominant phenolic compound in sea fennel, was determined by using high-performance liquid chromatography. The antioxidant activity of the samples was also tested by Ferric Reducing Antioxidant Power (FRAP) assay, and using two free radical scavenging methods using nitric oxide (NO•), and 2,2-diphenyl-1-picrilhydrazil radical (DPPH). From the obtained results it could be concluded that the sea fennel harvest location has great effect on the chemical composition and antioxidant activity of the extracts. Furthermore, the results point out the importance of the individual phenolics (chlorogenic acid) on the overall activity of the sea fennel extracts.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:364348
Study programmeTitle: Food technology - graduate - university
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjerka prehrambene tehnologije (magistar/magistra inženjerka prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-01-14 11:00:15