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undergraduate thesis
Kinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze
završni rad
2016. urn:nbn:hr:167:171366
Strunje, Kristina
University of Split
Faculty of Chemistry and Technology
Division of Chemistry

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Strunje, K. (2016). Kinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze : završni rad (Undergraduate thesis). Split: University of Split, Faculty of Chemistry and Technology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:171366

Strunje, Kristina. "Kinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:171366

Strunje, Kristina. "Kinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze : završni rad." Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, 2016. https://urn.nsk.hr/urn:nbn:hr:167:171366

Strunje, K. (2016). 'Kinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze : završni rad', Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, accessed 24 April 2024, https://urn.nsk.hr/urn:nbn:hr:167:171366

Strunje K. Kinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze : završni rad [Undergraduate thesis]. Split: University of Split, Faculty of Chemistry and Technology; 2016 [cited 2024 April 24] Available at: https://urn.nsk.hr/urn:nbn:hr:167:171366

K. Strunje, "Kinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze : završni rad", Undergraduate thesis, University of Split, Faculty of Chemistry and Technology, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:167:171366

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Metadata
TitleKinetičke značajke α-terpinolena kao inhibitora butirilkolinesteraze : završni rad
Title (english)Kinetic features of butyrylcholinesterase inhibitor α-terpinene : bachelor thesis
AuthorKristina Strunje
MentorFranko Burčul (mentor)
Committee memberFranko Burčul (predsjednik povjerenstva)
GranterUniversity of Split
Faculty of Chemistry and Technology
(Division of Chemistry)
Split
Defense date and country2016-10-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Enzimi su katalizatori u biološkim sustavima. Ubrzavaju reakcije snižavanjem energije aktivacije. Gotovo svi poznati enzimi su proteini. Najvažniji dio enzima je aktivno mjesto u kojem se odvija specifiĉna reakcija. Butirilkolinesteraza (BuChE) pripada skupini kolinesteraza odnosno estereaza. Esteraze spadaju u grupu hidrolaza. BuChE inaktivira neurotransmiter acetilkolin (ACh) i kao takva je postala terapeutski cilj u poodmaklim fazama Alzheimerove bolesti. Kao izvor enzima korištena je butirilkolinesteraza (BuChE) iz seruma konja, a kao supstrat enzima korišten je butiriltiokolin jodid (BuTChI). Kao inhibitor enzima korišten je α-terpinolen. Iz Lineweaver-Burk, Hanes-Wolf i Eisenthal – Cornish-Bowden prikaza te izraĉunatih postotaka inhibicije zakljuĉeno je da α-terpinolen pokazuje sposobnost inhibicije enzima BuChE. Kako se povećava koncentracija α-terpinolena povećava se i postotak inhibicije. Također je zakljuĉeno da se radi o inhibiciji miješanog tipa te su određeni odgovarajući kinetiĉki parametri KM, Vmax, KMapp, Vmaxapp, KI i KI'.
Abstract (english)
Enzymes are catalysts in biological systems. Accelerate reactions by lowering the activation energy. Nearly all known enzymes are proteins. The most important part of the enzyme is their active site where reactions take place. Butyrylcholinesterase (BuChE) belongs to a class of cholinesterase or esterase. Esterases belong to the group of hydrolases. BuChE inactivates the neurotransmitter acetylcholine (ACh) and as such has become a therapeutic target in later stages of Alzheimer's disease. Butyrylcholinesterase (BuChE) enzyme was obtained from the horse serum, and as a substrate of the enzyme was used butyrylthiocholine iodide (BuTChI). The enzyme used was α-terpinolene. From Lineweaver-Burk, Hanes-Wolf and Eisenthal - Cornish-Bowden display and calculated the percent inhibition was concluded that α-terpinolene shows the ability to inhibit the enzyme BuChE. α-Terpinolene inhibits BuChE in dose dependent manner. It was also concluded that it is a mixed type inhibition and certain corresponding kinetic parameters KM, Vmax, KMapp, Vmaxapp, KI and KI'.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:167:171366
ProjectNumber: 6897
Title: Istraživanje bioaktivnih spojeva iz dalmatinskog bilja: njihov antioksidacijski karakter i utjecaj na enzimsku inhibiciju i zdravlje
Acronym: BioActCom
Leader: Mladen Miloš
Jurisdiction: Croatia
Funder: HRZZ
Study programmeTitle: Chemistry
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteKljučne riječi doradio knjižničar / Key words have been revised by the librarian
Created on2017-05-30 10:45:21