Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor

Rogošić, Ante

prikaz prve stranice dokumenta Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor

master's thesis

Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor

diplomski rad

2017. urn:nbn:hr:167:909602

Rogošić, Ante

University of Split
Faculty of Chemistry and Technology
University of Split
School of Medicine

Cite this document

APA 6th Edition

Rogošić, A. (2017). Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad (Master's thesis). Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:167:909602

MLA 8th Edition

Rogošić, Ante. "Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad." Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:167:909602

Chicago 17th Edition

Harvard

Rogošić, A. (2017). 'Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad', Master's thesis, University of Split, Faculty of Chemistry and Technology; University of Split, School of Medicine, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:167:909602

Vancouver

Rogošić A. Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad [Master's thesis]. Split: University of Split, Faculty of Chemistry and Technology; Split: University of Split, School of Medicine; 2017 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:167:909602

IEEE

A. Rogošić, "Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad", Master's thesis, University of Split, Faculty of Chemistry and Technology, Split; University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:167:909602

Cite this item: https://urn.nsk.hr/urn:nbn:hr:167:909602

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Title	Razvoj i vrednovanje metode određivanja glutationa u farmaceutskim pripravcima slijednom analizom injektiranjem uz spektrofotometrijski detektor : diplomski rad
Title (english)	Sequential injection of glutathione in pharmaceutical formulations using spectrophotometric detector. Development and validation of the method. : diploma thesis
Author	Ante Rogošić
Mentor	Lea Kukoč Modun (mentor)
Committee member	Franko Burčul (predsjednik povjerenstva)
Committee member	Ivana Škugor Rončević (član povjerenstva)
Committee member	Lea Kukoč Modun (član povjerenstva)
Granter	University of Split Faculty of Chemistry and Technology Split
Granter	University of Split School of Medicine Split
Defense date and country	2017-10-27, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy
Abstract	U ovom je radu optimizirana i vrednovana metoda odreĎivanja glutationa slijednom analizom injektiranjem uz spektrofotometrijski detektor. Metoda se temelji na redoks-reakciji izmeĎu bakar(II)- neokuproin kompleksa i glutationa (GSH). Glutation ima ulogu reducensa te kao produkti nastaju oksidirani oblik glutationa (GSSG) i bakar(I)-neokuproin kompleks koji postiţe apsorpcijski maksimum pri λ = 458 nm. Optimizacijom parametara protočnog sustava obuhvaćeni su redoslijed injektiranja reagensa i uzorka, brzina protoka osnovne otopine, volumeni injektiranja analita i uzorka, duljina reakcijske uzvojnice te volumen petlje zadrţavanja. Tako optimiziranom metodom ostvareno je linerano dinamičko područje (LDP) u koncentracijskom rasponu od 6,00 × 10 ─7 mol L ─1 do 8,00 × 10 ─5 mol L ─1 uz pripadajuću jednadţbu pravca y = 3270 x – 0,0010 i koeficijent linearne regresije R 2 = 0,9983.Izmjerene su vrijednosti posluţile kao temelj za računanje granice dokazivanja (LOD = 2,20 × 10 ─7 mol L ─1 ) i granice odreĎivanja (LOQ = 6,00 × 10 ─7 mol L ─1 ). U odreĎivanju analita predloţenom metodom interferirali su: limunska kiselina, vinska kiselina, acetilsalicilna kiselina te natrijev citrat. Temeljem 15 uzastopnih injektiranja standardne otopine uzorka utvrĎena je ponovljivost metode uz relativno standardno odstupanje, RSD = 3,80 %. Mjerenjem smjesa otopina realnog uzorka i standardne otopine glutationa različitih koncentracija vrednovana je točnost metode. UsporeĎeni su rezultati odreĎivanja glutationa u realnom uzorku predloţenom metodom i metodom preporučenom farmakopejom. Slaganje rezultata (52,00 mg i 50,86 mg) upućuje na točnost metode.
Abstract (english)	A method for determination of glutathione using sequential injection analysis had been optimized and validated. The method is based on a redox reaction between glutathione and Cu(II)-neocuproine complex where glutathione is a reducens. Reaction products are oxidised form of glutathione (GSSG) and Cu(I)-neocuproine complex which reaches its absorption maximum at λ = 458 nm. Optimized parameters of flow system include sequence of injection of sample and reagent, rate of flow of carrier solution, volumes of injection of sample and reagent, volume of holding coil and lenght of reaction coil. Optimized method parameters were used to plot the callibration curve. The following equation had been determined: y = 3270 x – 0,0010. Linearity is achieved at analyte concentrations ranging from 6,00 × 10 ─7 mol L ─1 to 8,00 × 10 ─5 mol L ─1 . Limit of detection is LOD = 2,20 × 10 ─7 mol L ─1 while the limit of quantification is 6,00 × 10 ─7 mol L ─1 . Following compounds interfere: acetylsalicilyc acid, tartaric acid, citric acid and natrium citrate. Repeatability was confirmed based on results of 15 sequential injections of standard solution of glutathione. Relative standard deviation was 3,80 %. Method accuracy was validated by determining concentrations of mixed solutions composed of glutathione standard solution and glutathione real sample solution. Results of the developed method were compared with results of pharmacopoeial method. Similar results (52,00 g and 50,86 g) indicate method accuracy.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:167:909602
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Created on	2018-08-22 10:01:00